Reactive Oxygen Species (ROS) Fluorometric Assay Kit
BCA
Fluorometric
Cell Culture Cells, Tissue Samples
Quantitative
Catalog No. ABIN5708944
$324.00
Plus shipping costs $50.00
96 tests
Shipping to:
United States
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Quick Overview for Reactive Oxygen Species (ROS) Fluorometric Assay Kit (ABIN5708944)
Target
Reactive Oxygen Species (ROS)
Detection Method
Fluorometric
Application
Biochemical Assay (BCA)
Sample Type
Cell Culture Cells, Tissue Samples
Analytical Method
Quantitative
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Purpose
- This kit can be used for detection of ROS content in cells and tissue samples.
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Characteristics
- DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
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Material not included
- Fluorescence Microplate reader, Fluorescence Spectrophotometry, Flow Cytometry
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Assay Time
- 1 h
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- 4 °C,-20 °C
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Storage Comment
- This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
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Expiry Date
- 12 months
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- Reactive Oxygen Species (ROS)
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Alternative Name
- Reactive Oxygen Species
Target
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