Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

HSP70 1A ELISA Kit

HSPA1A Reactivity: Pig Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum
Catalog No. ABIN578822
  • Target See all HSP70 1A (HSPA1A) ELISA Kits
    HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
    Reactivity
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Pig
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156-10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Purpose
    This immunoassay kit allows for the in vitro quantitative determination of porcine Heat Shock Protein 70, HSP-70 concentrations in serum, plasma and other biological fluids.
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay recognizes recombinant and natural porcine HSP-70.
    Cross-Reactivity (Details)
    No significant cross-reactivity or interference was observed.
    Sensitivity
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Characteristics
    Sus scrofa,Pig,Heat shock 70 kDa protein 1A,Heat shock 70 kDa protein 1,HSP70.1,HSPA1A,HSPA1
    Components
    Reagent (Quantity ): Assay plate (1), Standard (2), Sample Diluent (1 × 20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1 × 120μl), Detection Reagent B (1 × 120μl), Wash Buffer (25 x concentrate) (1 × 30ml), Substrate (1x10ml), Stop Solution (1x10ml), Plate sealer for 96 wells (5), Instruction (1)
    Material not included
    Luminometer. Pipettes and pipette tips. EP tube Deionized or distilled water.
    Featured
    Discover our best selling HSPA1A ELISA Kit
    Top Product
    Discover our top product HSPA1A ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP-70. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HSP-70. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HSP-70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of HSP-70 in the samples is then 2 determined by comparing the O.D. of the samples to the standard curve.
    Reagent Preparation

    Bring all reagents to room temperature before use. Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. Dilute 30 mL of Wash Buffer Concentrate into deionized or distilled water to prepare 750 mL of Wash Buffer. Standard - Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 20 ng/ml. Allow the standard to sit for about 10 minutes with gentle agitation prior to making serial dilutions (Making serial dilution in the wells directly is not permitted). The undiluted standard serves as the highest standard (20 ng/ml). The Sample Diluent serves as the zero standard (0 ng/ml). ng/mL 20 10 5 2.5 1.25 0.625 0.312 0 Detection Reagent A and B - Dilute to the working concentration using Assay Diluent A or B (1:100), respectively.

    Sample Collection
    Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 20 minutes at approximately 1000 g. Remove serum and assay immediately or aliquot and store samples at -20 or -80 . Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 g at 2 - 8 within 30 minutes of collection. Store samples at -20 or -80 . Avoid repeated freeze-thaw cycles. Other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20 or -80 . Avoid repeated freeze-thaw cycles. Note: Serum and plasma to be used within 7 days may be stored at 2-8, otherwise samples must stored at -20 ( 1 month) or -80 ( 2 months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles. When performing the assay slowly bring samples to room temperature.
    Assay Procedure

    Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37 directly.). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at 4 until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    1. Add 100 of Standard, Blank, or Sample per well. Cover with the Plate sealer. Incubate for two hours at 37 .
    2. Remove the liquid of each well, don’t wash.
    3. Add 100 μl of Detection Reagent A working solution to each well. Cover with the Plate sealer. Incubate for 1 hour at 37 . Detection Reagent A working solution may appear cloudy. Warm to room temperature and mix gently until solution appears uniform.
    4. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash by filling each well with Wash Buffer (approximately 400 μl) using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher. Complete 4 removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
    5. Add 100 μl of Detection Reagent B working solution to each well. Cover with a new Plate sealer. Incubate for one hour at 37 .
    6. Repeat the aspiration/wash process for five times as conducted in step
    4. 7. Add 90 μl of Substrate Solution to each well. Cover with a new Plate sealer. Incubate for 15 - 30 minutes at 37 . Protect from light.
    8. Add 50 μl of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
    9. Determine the optical density of each well at once, using a microplate reader set to 450 nm.
    Important Note:1. Absorbance is a function of the incubation time. Therefore, prior to starting the assay it is recommended that all reagents should be freshly prepared prior to use and all required strip-wells are secured in the microtiter frame. This will ensure equal elapsed time for each pipetting step, without interruption.
    2. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. The reconstituted Standards can be used only once. This assay requires pipetting of small volumes. To minimize imprecision caused by pipetting, ensure that pipettors are calibrated. It is recommended to suck more than 10μl for once pipetting.
    3. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay.
    4. For each step in the procedure, total dispensing time for addition of reagents to the assay plate should not exceed 10 minutes.
    5. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
    6. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
    7. Duplication of all standards and specimens, although not required, is recommended.
    8. Substrate Solution is easily contaminated. Please protect it from light.

    Calculation of Results

    Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HSP-40 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation, such as curve expert 13.0. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

    Restrictions
    For Research Use only
  • Handling Advice
    1. The kit should not be used beyond the expiration date on the kit label.
    2. Do not mix or substitute reagents with those from other lots or sources.
    3. If samples generate values higher than the highest standard, further dilute the samples and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
    4. This assay is designed to eliminate interference by soluble receptors, ligands, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    5. Limited by the current condition and scientific technology, we can't completely conduct the comprehensive identification and analysis on the raw material provided by 3 suppliers. So there might be some qualitative and technical risks to use the kit.
    Storage
    4 °C/-20 °C
    Storage Comment
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Target See all HSP70 1A (HSPA1A) ELISA Kits
    HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))
    Alternative Name
    HSPA1A (HSPA1A Products)
    Synonyms
    hsp70-4 ELISA Kit, hspa1a ELISA Kit, Hsp70-3 ELISA Kit, Hsp70.3 ELISA Kit, Hsp72 ELISA Kit, hsp68 ELISA Kit, hsp70A1 ELISA Kit, HSP72 ELISA Kit, Hsp70-1 ELISA Kit, Hspa1 ELISA Kit, Hspa1b ELISA Kit, hsp70-1 ELISA Kit, hsp70-1a ELISA Kit, hsp70i ELISA Kit, hsp72 ELISA Kit, hspa1 ELISA Kit, hspa1b ELISA Kit, HSP70-1 ELISA Kit, HSP70-1A ELISA Kit, HSP70I ELISA Kit, HSPA1 ELISA Kit, HSPA1A ELISA Kit, HSPA1B ELISA Kit, HSP70-2 ELISA Kit, HSPA2 ELISA Kit, HSP70 ELISA Kit, hspA1A ELISA Kit, heat shock cognate 70-kd protein, tandem duplicate 3 ELISA Kit, heat shock 70 kDa protein 1 ELISA Kit, heat shock 70 kDa protein II ELISA Kit, heat shock protein 1A ELISA Kit, heat shock 70kD protein 1A ELISA Kit, heat shock protein family A (Hsp70) member 1A S homeolog ELISA Kit, heat shock protein family A (Hsp70) member 1A ELISA Kit, heat shock protein 70.2 ELISA Kit, heat shock 70kDa protein 1A ELISA Kit, heat shock 70 kDa protein 1B ELISA Kit, heat shock protein 70.1 ELISA Kit, hsp70.3 ELISA Kit, LOC100023597 ELISA Kit, LOC100554002 ELISA Kit, LOC100608888 ELISA Kit, Hspa1a ELISA Kit, hspa1a.S ELISA Kit, HSPA1A ELISA Kit, HSP70.2 ELISA Kit, LOC100354037 ELISA Kit, HSP70.1 ELISA Kit
    Background
    Heat shock proteins or HSPs are being synthesized under different kind of stress conditions and act as molecular chaperones for protein molecules. Because these proteins were first found in cells that were exposed to high temperatures, they are called ",heat shock proteins", and have been named according to their molecular weights. Usually HSPs are cytoplasmic proteins and they function in various intra-cellular processes. Heat shock proteins play an important role in protein-protein interactions, including folding and assisting in establishing of proper protein conformation, and prevention of inappropriate protein aggregation. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells, HSP72, a 72 kDa protein which is induced exclusively under stress conditions, HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles, GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum, and mitochondrial HSP70 (mtHSP70 , GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria.
    Gene ID
    3055
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
You are here:
Support