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Isocitrate Dehydrogenase Activity Colorimetric Assay Kit Kit

AcA Reactivity: Mammalian Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Samples
Pubmed (1)
Catalog No. ABIN593490
$395.00
Plus shipping costs $45.00
100 tests
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  • Target
    Isocitrate Dehydrogenase (IDH)
    Reactivity
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    Mammalian
    Detection Method
    Colorimetric
    Application
    Activity Assay (AcA)
    Sample Type
    Cell Culture Supernatant, Plasma, Serum, Tissue Samples
    Specificity
    It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in U/liter (U/L). In ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (lambdamax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well. Keywords: IDH, IDH assay
    Characteristics
    Isocitrate Dehydrogenase (IDH) Activity Assay Kit: Colorimetric Assay for Measuring IDH Activity in Serum, Tissues, Cells etc. Simple, Sensitive and Reliable.
    Detection method: Absorbance (450 nm), Sample type: Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc., Species reactivity: Mammalian, Application: This assay has a detection sensitivity as low as 0.01 mU. Features: Simple procedure, Fast and convenient, Sensitive assays for measuring IDH activity in various biological samples and the kit is also suitable for high throughput analyses.
    Components
    IDH Assay Buffer
    NADᶧ, (lyophilized)
    NADPᶧ, (lyophilized)
    IDH Substrate (lyophilized)
    Developer (lyophilized)
    IDH Positive Control (NADPᶧ,)
    NADH Standard (0.5 μmol, lyophilized)
  • Application Notes
    This assay has a detection sensitivity as low as 0.01 mU.
    Comment

    Further details regarding sample type: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Park, Lee, Han, Lee, Lee, Cho, Kim, Kim: "Toxic response of graphene nanoplatelets in vivo and in vitro." in: Archives of toxicology, 2014 (PubMed).

  • Target
    Isocitrate Dehydrogenase (IDH)
    Alternative Name
    Isocitrate Dehydrogenase
    Synonyms
    CG7176, CT22171, Dmel\\CG7176, IDH, IDH-NADP, Idh-NADP, idh, isocitrate dehydrogenase, l(3)L3852, F20D21.16, F20D21_16, SMU.672, icd, Isocitrate dehydrogenase, isocitrate dehydrogenase, isocitrate dehydrogenase, NADP-dependent, Monomeric isocitrate dehydrogenase, ankyrin repeat domain 26-like, Idh, ICDH, idh, icd, SSO_RS10660, JK_RS08640, LOC618625
    Background
    It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In
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