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CD31 ELISA Kit
PECAM1
Reactivity: Human
Colorimetric
Sandwich ELISA
15-6000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details CD31 ELISA Kit
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Target
See all CD31 (PECAM1) ELISA Kits
CD31 (PECAM1)
(Platelet/endothelial Cell Adhesion Molecule (PECAM1))
Reactivity
All reactivities for CD31 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
15-6000 pg/mL
Minimum Detection Limit
15 pg/mL
Application
ELISA
Purpose
Human PECAM-1 (CD31) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity
15 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples10 - 100 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for CD31
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Target
See all CD31 (PECAM1) ELISA Kits
CD31 (PECAM1)
(Platelet/endothelial Cell Adhesion Molecule (PECAM1))
Alternative Name
PECAM-1 (PECAM1 Products )
Synonyms
CD31 ELISA Kit, CD31/EndoCAM ELISA Kit, GPIIA' ELISA Kit, PECA1 ELISA Kit, PECAM-1 ELISA Kit, endoCAM ELISA Kit, C85791 ELISA Kit, Cd31 ELISA Kit, Pecam ELISA Kit, platelet/endothelial cell adhesion molecule 1 ELISA Kit, platelet and endothelial cell adhesion molecule 1 ELISA Kit, pecam1 ELISA Kit, PECAM1 ELISA Kit, Pecam1 ELISA Kit
Background
Gene Names: PECAM1 Protein names: Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA') (PECA1) (CD antigen CD31)
Gene ID
5175
UniProt
P16284
Pathways
Regulation of Actin Filament Polymerization
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