Resistin ELISA Kit

Catalog No. ABIN625082

Product Details Resistin ELISA Kit

Target: Resistin (RETN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2-400 pg/mL

Minimum Detection Limit: 2 pg/mL

Application: ELISA

Purpose: Human Resistin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples20 - 200 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for Resistin ELISA Kit (ABIN625082)

Siristatidis, Askoxylaki, Varounis, Kassanos, Chrelias: "E-selectin, resistin and reactive oxygen species levels in GnRH -agonist and -antagonist protocols in IVF/ICSI: a prospective cohort study." in: Journal of assisted reproduction and genetics, Vol. 32, Issue 6, pp. 959-67, (2016) (PubMed).

Erturk, Cure, Cure, Kurt, Cicek, Yuce: "Clinical potential of resistin as a novel prognostic biomarker for cellulitis." in: Experimental and therapeutic medicine, Vol. 9, Issue 5, pp. 1875-1880, (2015) (PubMed).

Nobili, Alisi, Cutrera, Carpino, De Stefanis, DOria, De Vito, Cucchiara, Gaudio, Musso: "Altered gut-liver axis and hepatic adiponectin expression in OSAS: novel mediators of liver injury in paediatric non-alcoholic fatty liver." in: Thorax, Vol. 70, Issue 8, pp. 769-81, (2015) (PubMed).

Pekta?, Kurt, Ün, Tiftik, Büyükaf?ar: "Effects of 17?-estradiol and progesterone on the production of adipokines in differentiating 3T3-L1 adipocytes: Role of Rho-kinase." in: Cytokine, Vol. 72, Issue 2, pp. 130-4, (2015) (PubMed).

Erturk, Cure, Parlak, Cure, Yuce, Kizilkaya: "Serum resistin levels may be new prognostic factor of crimean-congo hemorrhagic fever." in: International journal of clinical and experimental medicine, Vol. 7, Issue 10, pp. 3536-42, (2014) (PubMed).

Singh, Sridhar, Rajappa, Balachander, Kadhiravan: "Adiponectin-resistin index and its strong association with acute coronary syndrome in South Indian men." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 63, Issue 11, pp. 961-8, (2014) (PubMed).

Kahleova, Matoulek, Malinska, Oliyarnik, Kazdova, Neskudla, Skoch, Hajek, Hill, Kahle, Pelikanova: "Vegetarian diet improves insulin resistance and oxidative stress markers more than conventional diet in subjects with Type 2 diabetes." in: Diabetic medicine : a journal of the British Diabetic Association, Vol. 28, Issue 5, pp. 549-59, (2011) (PubMed).

Ojak: "[Cerebral changes in rats in postirradiation disease]." in: Patologia polska, Vol. 17, Issue 3, pp. 321-7 (PubMed).

Target Details for Resistin

Target: Resistin (RETN)

Alternative Name: Resistin (RETN Products)

Synonyms: RETN ELISA Kit, ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Fizz3 ELISA Kit, Rstn ELISA Kit, Xcp4 ELISA Kit, resistin ELISA Kit, RETN ELISA Kit, Retn ELISA Kit

Background: Gene Names: RETN FIZZ3 HXCP1 RSTN UNQ407/PRO1199
Protein names: Resistin (Adipose tissue-specific secretory factor) (ADSF) (C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein) (Cysteine-rich secreted protein A12-alpha-like 2) (Cysteine-rich secreted protein FIZZ3)

Gene ID: 56729

UniProt: Q9HD89

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration