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TGFA ELISA Kit

TGFA Reactivity: Human Colorimetric Sandwich ELISA 3-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625093
  • Target See all TGFA ELISA Kits
    TGFA (Transforming Growth Factor, alpha (TGFA))
    Reactivity
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    3-1000 pg/mL
    Minimum Detection Limit
    3 pg/mL
    Application
    ELISA
    Purpose
    Human TGF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ALCAM, Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, HB-EGF, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-13 Ra1, IL-18 BPa, IL-18 Rb, IL-18, IL-2 Ra, MMP-1, IL-2 Rb, IL-2 Rg, IL-21 R, MMP-2 , MMP-3, MMP-10, MMP-13).
    Sensitivity
    3 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our best selling TGFA ELISA Kit
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Lim, Yoo, Kim, Hur, Lee, Hur, Lee, Lee, Park, Lee, Chang, Kim, Kang, Hong, Kim, Kim, Yoon, Nam, Yang, Kim, Cho, Won: "GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands." in: Molecular cancer therapeutics, Vol. 15, Issue 2, pp. 251-63, (2016) (PubMed).

    Koob, Lim, Zabek, Massee: "Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing." in: Journal of biomedical materials research. Part B, Applied biomaterials, Vol. 103, Issue 5, pp. 1133-40, (2015) (PubMed).

    Briana, Liosi, Gourgiotis, Boutsikou, Marmarinos, Baka, Hassiakos, Malamitsi-Puchner: "Fetal concentrations of the growth factors TGF-α and TGF-β1 in relation to normal and restricted fetal growth at term." in: Cytokine, Vol. 60, Issue 1, pp. 157-61, (2013) (PubMed).

    Miyabayashi, Ijichi, Mohri, Tada, Yamamoto, Asaoka, Ikenoue, Tateishi, Nakai, Isayama, Morishita, Omata, Moses, Koike: "Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals." in: Cancer research, Vol. 73, Issue 7, pp. 2221-34, (2013) (PubMed).

    Drzymała-Czyż, Banasiewicz, Tubacka, Majewski, Biczysko, Kościński, Drews, Walkowiak: "Inulin supplementation in rat model of pouchitis." in: Acta biochimica Polonica, Vol. 58, Issue 3, pp. 381-4, (2012) (PubMed).

    Franzke, Cobzaru, Triantafyllopoulou, Löffek, Horiuchi, Threadgill, Kurz, van Rooijen, Bruckner-Tuderman, Blobel: "Epidermal ADAM17 maintains the skin barrier by regulating EGFR ligand-dependent terminal keratinocyte differentiation." in: The Journal of experimental medicine, Vol. 209, Issue 6, pp. 1105-19, (2012) (PubMed).

    Pinto, Di Fabio, Maiello, Pini, Latiano, Aschele, Garufi, Bochicchio, Rosati, Aprile, Giaquinta, Torri, Bardelli, Gion, Martoni: "Phase II study of panitumumab, oxaliplatin, 5-fluorouracil, and concurrent radiotherapy as preoperative treatment in high-risk locally advanced rectal cancer patients (StarPan/STAR-02 Study)." in: Annals of oncology : official journal of the European Society for Medical Oncology, Vol. 22, Issue 11, pp. 2424-30, (2011) (PubMed).

    Acocella, Bertrand, Beytout, Durrande, Garcia Rodriguez, Kosmidis, Micoud, Rey, Rodriguez Zapata, Roux: "Comparison of three different regimens in the treatment of acute brucellosis: a multicenter multinational study." in: The Journal of antimicrobial chemotherapy, Vol. 23, Issue 3, pp. 433-9, (1989) (PubMed).

  • Target See all TGFA ELISA Kits
    TGFA (Transforming Growth Factor, alpha (TGFA))
    Alternative Name
    TGF-alpha (TGFA Products)
    Synonyms
    TFGA ELISA Kit, LOC100009482 ELISA Kit, TGF alpha ELISA Kit, TGFA ELISA Kit, wa-1 ELISA Kit, wa1 ELISA Kit, RATTGFAA ELISA Kit, TGFAA ELISA Kit, NV15606 ELISA Kit, si:ch211-10e8.7 ELISA Kit, transforming growth factor alpha ELISA Kit, transforming growth factor alpha L homeolog ELISA Kit, transforming growth factor-alpha ELISA Kit, transforming growth factor, alpha ELISA Kit, TGFA ELISA Kit, TGF-A ELISA Kit, Tgfa ELISA Kit, tgfa.L ELISA Kit, LOC100121300 ELISA Kit, LOC100009150 ELISA Kit, tgfa ELISA Kit
    Background
    Gene Names: TGFA
    Protein names: Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha (TGF-alpha) (EGF-like TGF) (ETGF) (TGF type 1)]
    Gene ID
    7039
    UniProt
    P01135
    Pathways
    NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway
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