TNFRSF10A ELISA Kit

Catalog No. ABIN625358

Product Details TNFRSF10A ELISA Kit

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 40-10.000 pg/mL

Minimum Detection Limit: 40 pg/mL

Application: ELISA

Purpose: Custom Human TRAIL Receptor 1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes human TRAIL R1.

Sensitivity: 40 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for TNFRSF10A

Target: TNFRSF10A (Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))

Alternative Name: TRAIL R1 (TNFRSF10A Products)

Synonyms: APO2 ELISA Kit, CD261 ELISA Kit, DR4 ELISA Kit, TRAILR-1 ELISA Kit, TRAILR1 ELISA Kit, TNFRSF10A ELISA Kit, TNF receptor superfamily member 10a ELISA Kit, tumor necrosis factor receptor superfamily member 26 ELISA Kit, tumor necrosis factor receptor superfamily, member 10a ELISA Kit, TNFRSF10A ELISA Kit, LOC510798 ELISA Kit

Background: Gene Names: TNFSF10 APO2L TRAIL
Protein names: Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)

Gene ID: 8797

UniProt: O00220

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity