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TNFRSF19 ELISA Kit

TNFRSF19 Reactivity: Human Colorimetric Sandwich ELISA 50 pg/mL-400 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625364
  • Target See all TNFRSF19 ELISA Kits
    TNFRSF19 (Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))
    Reactivity
    • 4
    • 3
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    50 pg/mL-400 ng/mL
    Minimum Detection Limit
    50 pg/mL
    Application
    ELISA
    Purpose
    Human TROY (TNFRSF19) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensitivity
    50 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product TNFRSF19 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 - 10 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all TNFRSF19 ELISA Kits
    TNFRSF19 (Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))
    Alternative Name
    TROY (TNFRSF19 Products)
    Synonyms
    TNFRSF19 ELISA Kit, DKFZp468A0320 ELISA Kit, TAJ ELISA Kit, TAJ-alpha ELISA Kit, TRADE ELISA Kit, TROY ELISA Kit, AL023044 ELISA Kit, AW123854 ELISA Kit, TAJ-ALPHA ELISA Kit, Troy ELISA Kit, RGD1564996 ELISA Kit, TNF receptor superfamily member 19 ELISA Kit, tumor necrosis factor receptor superfamily, member 19 ELISA Kit, TNFRSF19 ELISA Kit, Tnfrsf19 ELISA Kit
    Background
    Gene Names: TNFRSF19 TAJ TROY UNQ1888/PRO4333
    Protein names: Tumor necrosis factor receptor superfamily member 19 (TRADE) (Toxicity and JNK inducer)
    Gene ID
    55504
    UniProt
    Q9NS68
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