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CD25 ELISA Kit

IL2RA Reactivity: Mouse Colorimetric Sandwich ELISA 10-2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN625409
  • Target See all CD25 (IL2RA) ELISA Kits
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Reactivity
    • 7
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    10-2500 pg/mL
    Minimum Detection Limit
    10 pg/mL
    Application
    ELISA
    Purpose
    Mouse IL-2 R alpha ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes mouse IL-2 R alpha.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples5 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all CD25 (IL2RA) ELISA Kits
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Alternative Name
    IL-2 R alpha (IL2RA Products)
    Synonyms
    CD25 ELISA Kit, IDDM10 ELISA Kit, IL2R ELISA Kit, TCGFR ELISA Kit, IL2RAC ELISA Kit, IL-2RA ELISA Kit, Il2r ELISA Kit, Ly-43 ELISA Kit, interleukin 2 receptor subunit alpha ELISA Kit, interleukin-2 receptor subunit alpha-like ELISA Kit, interleukin 2 receptor, alpha chain ELISA Kit, IL2RA ELISA Kit, Il2ra ELISA Kit, LOC100222957 ELISA Kit
    Gene ID
    16184
    UniProt
    P01590
    Pathways
    JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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