CCL17 ELISA Kit

Catalog No. ABIN625429

Product Details CCL17 ELISA Kit

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 4-3000 pg/mL

Minimum Detection Limit: 4 pg/mL

Application: ELISA

Purpose: Mouse TARC (CCL17) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-2, MIP-3 alpha, MIP-3 beta, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

Sensitivity: 4 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CCL17

Target: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Alternative Name: TARC (CCL17 Products)

Synonyms: A-152E5.3 ELISA Kit, ABCD-2 ELISA Kit, SCYA17 ELISA Kit, TARC ELISA Kit, CCL17 ELISA Kit, Abcd-2 ELISA Kit, Scya17 ELISA Kit, Scya17l ELISA Kit, Tarc ELISA Kit, abcd2 ELISA Kit, C-C motif chemokine ligand 17 ELISA Kit, chemokine (C-C motif) ligand 17 ELISA Kit, ATP-binding cassette sub-family D member 2 ELISA Kit, CCL17 ELISA Kit, Ccl17 ELISA Kit, LOC100537809 ELISA Kit

Background: Gene Names: Ccl17 ABCD-2 TARC mCG_12286
Protein names: C-C motif chemokine

Gene ID: 20295

UniProt: Q9WUZ6