CD13 IQ-ELISA Kit

Catalog No. ABIN6385778

Product Details CD13 IQ-ELISA Kit

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Human

Detection Method: qPCR

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Immunoquantitative (PCR-Based) Aminopeptidase N (ANPEP) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensitivity: 4 pg/mL

Characteristics: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Components:

• Aminopeptidase N (ANPEP) Microplate (Item A): 96 well PCR plate coated with anti-Human Aminopeptidase N (ANPEP)
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Aminopeptidase N (ANPEP)
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Aminopeptidase N (ANPEP) (Item F): 2 vials of a 4x concentrated solution of anti-Human Aminopeptidase N (ANPEP) affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Material not included:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Application Details

Application Notes: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Comment:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Sample Volume: 25 µL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: Aminopeptidase N (ANPEP) (ANPEP Products)

Synonyms: APN IQ-ELISA Kit, CD13 IQ-ELISA Kit, GP150 IQ-ELISA Kit, LAP1 IQ-ELISA Kit, P150 IQ-ELISA Kit, PEPN IQ-ELISA Kit, A2AP IQ-ELISA Kit, AAP IQ-ELISA Kit, ALPHA-2-PI IQ-ELISA Kit, API IQ-ELISA Kit, PLI IQ-ELISA Kit, AAT1 IQ-ELISA Kit, ALT1 IQ-ELISA Kit, GPT1 IQ-ELISA Kit, Gpt1 IQ-ELISA Kit, Apn IQ-ELISA Kit, pepN IQ-ELISA Kit, SC8E4A.13 IQ-ELISA Kit, 1300007J06Rik IQ-ELISA Kit, 2310022B03Rik IQ-ELISA Kit, ALT IQ-ELISA Kit, Gpt-1 IQ-ELISA Kit, AP-M IQ-ELISA Kit, AP-N IQ-ELISA Kit, Cd13 IQ-ELISA Kit, Apm IQ-ELISA Kit, KZP IQ-ELISA Kit, Lap1 IQ-ELISA Kit, rAPN IQ-ELISA Kit, APDE IQ-ELISA Kit, p150 IQ-ELISA Kit, anpep IQ-ELISA Kit, fb64c05 IQ-ELISA Kit, wu:fb64c05 IQ-ELISA Kit, zgc:136771 IQ-ELISA Kit, alanyl aminopeptidase, membrane IQ-ELISA Kit, serpin family F member 2 IQ-ELISA Kit, glutamic--pyruvic transaminase IQ-ELISA Kit, aminopeptidase N IQ-ELISA Kit, aminopeptidase IQ-ELISA Kit, aminopeptidase n IQ-ELISA Kit, glutamic pyruvic transaminase, soluble IQ-ELISA Kit, alanyl (membrane) aminopeptidase IQ-ELISA Kit, membrane alanyl aminopeptidase IQ-ELISA Kit, alanyl (membrane) aminopeptidase b IQ-ELISA Kit, alanyl aminopeptidase, membrane L homeolog IQ-ELISA Kit, ANPEP IQ-ELISA Kit, SERPINF2 IQ-ELISA Kit, GPT IQ-ELISA Kit, Gpt IQ-ELISA Kit, Apn2 IQ-ELISA Kit, Apn1 IQ-ELISA Kit, SCO2643 IQ-ELISA Kit, pepN IQ-ELISA Kit, Shewana3_0897 IQ-ELISA Kit, Alvin_1024 IQ-ELISA Kit, Ndas_3413 IQ-ELISA Kit, Saut_1544 IQ-ELISA Kit, Fbal_1877 IQ-ELISA Kit, ML5_3424 IQ-ELISA Kit, Despr_1988 IQ-ELISA Kit, Mesop_5365 IQ-ELISA Kit, Anpep IQ-ELISA Kit, LOC101744250 IQ-ELISA Kit, anpepb IQ-ELISA Kit, anpep.L IQ-ELISA Kit

Gene ID: 290

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones