CD137 ELISA Kit

Catalog No. ABIN6719707

Product Details CD137 ELISA Kit

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: 4-1BB, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, Homolog of mouse 4 1BB, ILA, Induced by lymphocyte activation (ILA), Interleukin activated receptor homolog of mouse Ly63, Receptor protein 4 1BB, T-cell antigen 4-1BB homolog, T-cell antigen ILA, TNFRSF9, TNR9_HUMAN, Tumor necrosis factor receptor superfamily member 9

Background: CD137 is a member of the tumor necrosis factor(TNF) receptor family. Its alternative names are tumor necrosis factor receptor superfamily member 9(TNFRSF9), 4-1BB and induced by lymphocyte activation(ILA). CD137 gene was localized to chromosome 1p36.Members of this receptor family and their structurally related ligands are important regulators of a wide variety of physiological processes and play an especially important role in the regulation of immune responses. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.

Gene Name: TNFRSF9

Production: Natural and recombinant mouse TNFRSF9. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V24-L187

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TNFRSF9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TNFRSF9 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD137

Target: CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))

Alternative Name: TNFRSF9 - 4-1BB (TNFRSF9 Products)

Synonyms: TNFRSF9 ELISA Kit, zgc:136557 ELISA Kit, 4-1BB ELISA Kit, CD137 ELISA Kit, CDw137 ELISA Kit, ILA ELISA Kit, A930040I11Rik ELISA Kit, AA408498 ELISA Kit, AI325004 ELISA Kit, Cd137 ELISA Kit, Ly63 ELISA Kit, tumor necrosis factor receptor superfamily member 9 ELISA Kit, TNF receptor superfamily member 9 ELISA Kit, tumor necrosis factor receptor superfamily, member 9a ELISA Kit, tumor necrosis factor receptor superfamily, member 9 ELISA Kit, TNFRSF9 ELISA Kit, tnfrsf9a ELISA Kit, Tnfrsf9 ELISA Kit

Gene ID: 21942

NCBI Accession: NP_001070977

UniProt: P20334

Pathways: Cancer Immune Checkpoints