GAL4 ELISA Kit
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- Target See all GAL4 (LGALS4) ELISA Kits
- GAL4 (LGALS4) (Galectin 4 (LGALS4))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 156 pg/mL - 10000 pg/mL
- Minimum Detection Limit
- 156 pg/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Characteristics
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Synonyms: Antigen NY-CO-27, Gal-4, Galectin-4, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4, LEG4_HUMAN, LGALS4
Background: Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
Gene Name: LGALS4
Production: Natural and recombinant human Galectin-4. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: E.coli, Immunogen sequence: A2-I323
- Top Product
- Discover our top product LGALS4 ELISA Kit
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- Application Notes
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-4 standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10,000pg/mL
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Target See all GAL4 (LGALS4) ELISA Kits
- GAL4 (LGALS4) (Galectin 4 (LGALS4))
- Alternative Name
- Galectin-4 (LGALS4 Products)
- Synonyms
- LGALS4 ELISA Kit, gal4 ELISA Kit, l36lbp ELISA Kit, MGC86186 ELISA Kit, galectin-4 ELISA Kit, xgalectin-VIa ELISA Kit, gal-4 ELISA Kit, L36LBP ELISA Kit, L-36 ELISA Kit, L36LBI ELISA Kit, GAL4 ELISA Kit, galectin 4 ELISA Kit, lectin, galactoside-binding, soluble, 4 L homeolog ELISA Kit, Galectin-4 ELISA Kit, lectin, galactose binding, soluble 4 ELISA Kit, LGALS4 ELISA Kit, lgals4.L ELISA Kit, leg4 ELISA Kit, Lgals4 ELISA Kit
- Gene ID
- 3960
- NCBI Accession
- NP_006140
- UniProt
- P56470
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