PEDF ELISA Kit
-
- Target See all PEDF (SERPINF1) ELISA Kits
- PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Minimum Detection Limit
- 0.78 ng/mL
- Application
- ELISA
- Purpose
- Sandwich ELISA for Quantitative Detection of Antigen
- Sample Type
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Characteristics
-
Synonyms: Alpha 2 antiplasmin, Cell proliferation-inducing gene 35 protein, EPC1, OI12, OI6, PEDF, PEDF_HUMAN, PIG35, Pigment epithelium derived factor, Pigment epithelium-derived factor, Proliferation inducing protein 35, serine (or cysteine) proteinase inhibitor clade F (alpha-2 antiplasmin, pigment epithelium derived factor) member 1, SERPIN F1, Serpin peptidase inhibitor clade F member , |serpin peptidase inhibitor clade F (alpha-2 antiplasmin pigment epithelium derived factor) member 1, SERPINF1
Background: Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
Gene Name: SERPINF1
Production: Natural and recombinant human PEDF. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: M1-P418
- Top Product
- Discover our top product SERPINF1 ELISA Kit
-
-
- Application Notes
-
Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human PEDF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human PEDF standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 0.78 ng/mL-50 ng/mL
Other Performance Data: DOE: 2-8° C - 12/25/18 -20° C - 6/25/19
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
-
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
-
- Target See all PEDF (SERPINF1) ELISA Kits
- PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
- Alternative Name
- PEDF - SerpinF1 (SERPINF1 Products)
- Synonyms
- EPC-1 ELISA Kit, OI12 ELISA Kit, OI6 ELISA Kit, PEDF ELISA Kit, MGC82984 ELISA Kit, SERPINF1 ELISA Kit, AI195227 ELISA Kit, Pedf ELISA Kit, Pedfl ELISA Kit, Sdf3 ELISA Kit, Dmrs91 ELISA Kit, zgc:92072 ELISA Kit, serpin family F member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 1 ELISA Kit, SERPINF1 ELISA Kit, serpinf1 ELISA Kit, serpinf1.L ELISA Kit, Serpinf1 ELISA Kit
- Gene ID
- 5176
- NCBI Accession
- NP_002606
- UniProt
- P36955
-