TNFSF13 ELISA Kit

Catalog No. ABIN6720117

Supplier Product No.: koa0557

This Human TNFSF13 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human TNFSF13.

Quick Overview for TNFSF13 ELISA Kit (ABIN6720117)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details TNFSF13 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0557

Supplier: Rockland

Purpose: Human TNFSF13 - APRIL Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human TNFSF13. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 156 pg/mL-10,000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TNFSF13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TNFSF13 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A105-L250

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: TNFSF13/APRIL

Background:

Synonyms: A proliferation-inducing ligand, APRIL, CD256, TALL-2, TALL2, TNF- and APOL-related leukocyte expressed ligand 2, TNF-related death ligand 1, TNF13_HUMAN, TNFSF13, TRDL-1, Tumor necrosis factor (ligand) superfamily member 13, Tumor necrosis factor ligand superfamily member 13, Tumor necrosis factor like protein ZTNF2, Tumor necrosis factor related death ligand 1, ZTNF2

Background: Tumor necrosis factor ligand superfamily member 13(TNFSF13) also known as a proliferation-inducing ligand(APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256(cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand(TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.

Gene ID: 8741

NCBI Accession: NP_001185551

UniProt: Q2QBA2

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response