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ANG ELISA Kit

ANG Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6720265
  • Target See all ANG ELISA Kits
    ANG (Angiogenin (ANG))
    Reactivity
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: ALS9, ANG, ANG 1, ANG I, ANG1, ANGI, Angiogenin ribonuclease RNase A family 5, Epididymis luminal protein 168, HEL168, MGC22466, MGC71966, Ribonuclease 5, Ribonuclease RNase A Family 5, Ribonuclease5, RNASE 5, RNASE4, RNASE5

    Background: Angiogenin(Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al.(2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al.(1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

    Gene Name: ANG

    Production: Natural and recombinant human ANG. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: Q25-P147

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  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human ANG standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANG standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 78pg/mL-5000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all ANG ELISA Kits
    ANG (Angiogenin (ANG))
    Alternative Name
    ANG (ANG Products)
    Synonyms
    ALS9 ELISA Kit, HEL168 ELISA Kit, RNASE4 ELISA Kit, RNASE5 ELISA Kit, ANG ELISA Kit, AI385586 ELISA Kit, Ang1 ELISA Kit, Rnase5 ELISA Kit, Rnase5a ELISA Kit, ANG1 ELISA Kit, angiogenin ELISA Kit, angiogenin, ribonuclease, RNase A family, 5 ELISA Kit, ribonuclease A family member k6 ELISA Kit, ANG ELISA Kit, Ang ELISA Kit, RNASE6 ELISA Kit
    Gene ID
    283
    NCBI Accession
    NP_001091046
    UniProt
    P03950
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