IGFBP7 ELISA Kit

Catalog No. ABIN6730695

Product Details IGFBP7 ELISA Kit

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.12 ng/mL - 25 ng/mL

Minimum Detection Limit: 0.12 ng/mL

Application: ELISA

Purpose: Mouse IGFBP-rp1/IGFBP-7 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse IGFBP-rp1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samples20 fold

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IGFBP7

Target: IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))

Alternative Name: IGFBP-rp1 (IGFBP-7 (IGFBP7 Products)

Synonyms: zgc:85888 ELISA Kit, AGM ELISA Kit, FSTL2 ELISA Kit, IBP-7 ELISA Kit, IGFBP-7 ELISA Kit, IGFBP-7v ELISA Kit, IGFBPRP1 ELISA Kit, MAC25 ELISA Kit, PSF ELISA Kit, RAMSVPS ELISA Kit, TAF ELISA Kit, Fstl2 ELISA Kit, Mac25 ELISA Kit, insulin like growth factor binding protein 7 ELISA Kit, insulin-like growth factor binding protein 7 ELISA Kit, IGFBP7 ELISA Kit, igfbp7 ELISA Kit, Igfbp7 ELISA Kit

UniProt: Q61581

Pathways: Growth Factor Binding