Phospholipase C gamma 1 ELISA Kit

Catalog No. ABIN6730786

Product Details Phospholipase C gamma 1 ELISA Kit

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human, Mouse and Rat Phospho-PLCG1 (Tyr771) and Total PLCG1 ELISA Kit. This assay semi-quantitatively measures phosphorylated PLCG1 (Tyr771) and Total PLCG1 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes Human, Mouse, and Rat PLCG1 (Tyr771)

Characteristics:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCG1 (PLCG1 Products)

Synonyms: NCKAP3 ELISA Kit, PLC-II ELISA Kit, PLC1 ELISA Kit, PLC148 ELISA Kit, PLCgamma1 ELISA Kit, plc1 ELISA Kit, nckap3 ELISA Kit, plc-ii ELISA Kit, plc148 ELISA Kit, plcgamma1 ELISA Kit, PPLCA ELISA Kit, AI894140 ELISA Kit, Cded ELISA Kit, Plc-1 ELISA Kit, Plc-gamma1 ELISA Kit, Plcg-1 ELISA Kit, fj46f04 ELISA Kit, wu:fj46f04 ELISA Kit, wu:fj57a05 ELISA Kit, phospholipase C gamma 1 ELISA Kit, phospholipase C gamma 1 L homeolog ELISA Kit, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 ELISA Kit, phospholipase C, gamma 1 ELISA Kit, PLCG1 ELISA Kit, plcg1.L ELISA Kit, LOC100633163 ELISA Kit, Plcg1 ELISA Kit, plcg1 ELISA Kit

Gene ID: 5335

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling