MST1 ELISA Kit

Catalog No. ABIN6730827

Product Details MST1 ELISA Kit

Target: MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse MSP ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse MSP.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for MST1

Target: MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))

Alternative Name: MSP (MST1 Products)

Synonyms: D3F15S2 ELISA Kit, DNF15S2 ELISA Kit, HGFL ELISA Kit, MSP ELISA Kit, NF15S2 ELISA Kit, E2F2 ELISA Kit, D3F15S2h ELISA Kit, D9H3F15S2 ELISA Kit, DNF15S2h ELISA Kit, Hgfl ELISA Kit, HGF1/MSP ELISA Kit, E1A ELISA Kit, XHL ELISA Kit, d3f15s2 ELISA Kit, dnf15s2 ELISA Kit, hgfl ELISA Kit, msp ELISA Kit, mst1 ELISA Kit, nf15s2 ELISA Kit, macrophage stimulating 1 ELISA Kit, macrophage stimulating 1 (hepatocyte growth factor-like) ELISA Kit, serine/threonine kinase 4 ELISA Kit, macrophage stimulating 1 L homeolog ELISA Kit, MST1 ELISA Kit, Mst1 ELISA Kit, STK4 ELISA Kit, mst1.L ELISA Kit

UniProt: P26928