alpha Tubulin ELISA Kit

Catalog No. ABIN6810527

Product Details alpha Tubulin ELISA Kit

Target: alpha Tubulin (TUBA1)

Binding Specificity: acetylated

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human, Mouse, Rat Acetylated-Alpha Tubulin ELISA Kit. This ELISA is for measuring Acetylated-Alpha Tubulin in Human, Mouse, Rat cell lysates.

Sample Type: Cell Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes Human///Mouse///Rat Acetylated-Alpha Tubulin.

Characteristics:

• Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-cleaved Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin (TUBA1 Products)

Synonyms: Tuba1 ELISA Kit, K-ALPHA-1 ELISA Kit, Tuba-1 ELISA Kit, cb944 ELISA Kit, fb22g06 ELISA Kit, tuba1 ELISA Kit, wu:fb22g06 ELISA Kit, Calpha1 ELISA Kit, tubulin, alpha 1A ELISA Kit, tubulin alpha 1b ELISA Kit, tubulin, alpha 1b ELISA Kit, tubulin, alpha 1B ELISA Kit, Tuba1a ELISA Kit, TUBA1B ELISA Kit, tuba1b ELISA Kit, Tuba1b ELISA Kit

Gene ID: 7846

UniProt: Q71U36

Pathways: Microtubule Dynamics