TNFRSF11A ELISA Kit
TNFRSF11A
Reactivity: Rat
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
Target
See all TNFRSF11A ELISA Kits
-
TNFRSF11A
(Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
-
Reactivity
-
Rat
-
Detection Method
-
Colorimetric
-
Method Type
-
Sandwich ELISA
-
Application
-
ELISA
-
Purpose
-
Rat RANK/TNFRSF11A ELISA Kit.
-
Sample Type
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytical Method
-
Quantitative
-
Characteristics
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Components
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Material not included
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
Top Product
-
Discover our top product TNFRSF11A ELISA Kit
-
-
-
Application Notes
-
Optimal working dilution should be determined by the investigator.
-
Plate
-
Pre-coated
-
Protocol
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
-
Restrictions
-
For Research Use only
-
-
Expiry Date
-
6 months
-
-
Target
See all TNFRSF11A ELISA Kits
-
TNFRSF11A
(Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
-
Alternative Name
-
RANK (TNFRSF11A (TNFRSF11A Products)
-
Synonyms
-
RANK ELISA Kit, RGD1563614 ELISA Kit, TNFRSF11A ELISA Kit, Ly109 ELISA Kit, ODFR ELISA Kit, OFE ELISA Kit, Rank ELISA Kit, TRANCE-R ELISA Kit, mRANK ELISA Kit, CD265 ELISA Kit, FEO ELISA Kit, LOH18CR1 ELISA Kit, OPTB7 ELISA Kit, OSTS ELISA Kit, PDB2 ELISA Kit, TRANCER ELISA Kit, TNF receptor superfamily member 11A ELISA Kit, TNF receptor superfamily member 11a ELISA Kit, tumor necrosis factor receptor superfamily, member 11a, NFKB activator ELISA Kit, Tnfrsf11a ELISA Kit, TNFRSF11A ELISA Kit
-
Gene ID
-
498206
-
UniProt
-
F1M8Z6
-
Pathways
-
NF-kappaB Signaling
-