DC-SIGN/CD209 ELISA Kit

Catalog No. ABIN6951432

Product Details DC-SIGN/CD209 ELISA Kit

Target: DC-SIGN/CD209 (CD209) (CD209)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse DC-SIGN ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for DC-SIGN/CD209

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: DC-SIGN (CD209 Products)

Synonyms: CDSIGN ELISA Kit, CLEC4L ELISA Kit, DC-SIGN ELISA Kit, DC-SIGN1 ELISA Kit, cd209 ELISA Kit, CLEC4M ELISA Kit, si:ch211-224h1.3 ELISA Kit, CD209 ELISA Kit, CIRE ELISA Kit, Dcsign ELISA Kit, SIGN-R1 ELISA Kit, SIGNR5 ELISA Kit, Cd209 ELISA Kit, CD209 molecule ELISA Kit, CD209a antigen ELISA Kit, CD209 antigen-like protein D ELISA Kit, CD209c molecule ELISA Kit, CD209 antigen ELISA Kit, CD209 ELISA Kit, cd209 ELISA Kit, Cd209a ELISA Kit, LOC100529184 ELISA Kit, Cd209c ELISA Kit, LOC100460708 ELISA Kit, LOC105484282 ELISA Kit

Gene ID: 170786

UniProt: Q91ZX1