RCAS1 ELISA Kit

Catalog No. ABIN6951439

Product Details RCAS1 ELISA Kit

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human EBAG9 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for RCAS1

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Alternative Name: EBAG9 (EBAG9 Products)

Synonyms: EB9 ELISA Kit, PDAF ELISA Kit, MGC56608 ELISA Kit, zgc:56608 ELISA Kit, MGC84425 ELISA Kit, EBAG9 ELISA Kit, AI835379 ELISA Kit, Rcas1 ELISA Kit, RCAS1 ELISA Kit, eb9 ELISA Kit, pdaf ELISA Kit, rcas1 ELISA Kit, estrogen receptor binding site associated, antigen, 9 ELISA Kit, estrogen receptor binding site associated, antigen, 9 L homeolog ELISA Kit, estrogen receptor-binding fragment-associated gene 9 ELISA Kit, EBAG9 ELISA Kit, ebag9 ELISA Kit, ebag9.L ELISA Kit, Ebag9 ELISA Kit

Gene ID: 9166

UniProt: O00559