IKKi/IKKe ELISA Kit

Catalog No. ABIN6951456

Product Details IKKi/IKKe ELISA Kit

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human IKK-E/IKBKE ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for IKKi/IKKe

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Alternative Name: IKK-E (IKBKE (IKBKE Products)

Synonyms: zgc:100844 ELISA Kit, IKBKE ELISA Kit, MGC130907 ELISA Kit, IKK-E ELISA Kit, IKK-i ELISA Kit, IKKE ELISA Kit, IKKI ELISA Kit, AW558201 ELISA Kit, IKKepsilon ELISA Kit, Ikki ELISA Kit, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit epsilon ELISA Kit, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog ELISA Kit, inhibitor of kappaB kinase epsilon ELISA Kit, IKBKE ELISA Kit, ikbke ELISA Kit, ikbke.L ELISA Kit, Ikbke ELISA Kit

Gene ID: 9641

UniProt: Q14164

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades