TNFSF9 ELISA Kit (Tumor Necrosis Factor (Ligand) Superfamily, Member 9)

Details for Product TNFSF9 ELISA Kit No. ABIN6951520
Target
Reactivity
Rat
Alternatives
Kits with alternative reactivity to:
6
2
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Options
Purpose Rat 4-1BB Ligand/TNFSF9 ELISA Kit.
Sample Type Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Target
Alternative Name 4-1BB Ligand (TNFSF9 (TNFSF9 ELISA Kit Abstract)
Gene ID 353218
UniProt Q80WE6
Pathways Activated T Cell Proliferation, Cancer Immune Checkpoints
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Expiry Date 6 months