ITCH ELISA Kit

Catalog No. ABIN6951536

Product Details ITCH ELISA Kit

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ITCH ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for ITCH

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Alternative Name: ITCH (ITCH Products)

Synonyms: AIF4 ELISA Kit, AIP4 ELISA Kit, NAPP1 ELISA Kit, dJ468O1.1 ELISA Kit, 6720481N21Rik ELISA Kit, 8030492O04Rik ELISA Kit, A130065M08 ELISA Kit, C230047C07Rik ELISA Kit, itchy E3 ubiquitin protein ligase ELISA Kit, itchy, E3 ubiquitin protein ligase ELISA Kit, ITCH ELISA Kit, Itch ELISA Kit

Gene ID: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events