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Beclin 1 ELISA Kit

BECN1 Reactivity: Human, Rat, Mouse pSer234 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN6951539
  • Target See all Beclin 1 (BECN1) ELISA Kits
    Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
    Binding Specificity
    pSer234
    Reactivity
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human/Mouse/Rat Phospho-Beclin-1 (S234) and Total Beclin-1 ELISA Kit. This assay semi-quantitatively measures phosphorylated Beclin-1 (Ser234) and Total Beclin-1 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human, mouse, and rat Beclin-1 phosphorylated at site Serine-234 and Total Beclin-1.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all Beclin 1 (BECN1) ELISA Kits
    Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))
    Alternative Name
    Beclin-1 (BECN1 Products)
    Synonyms
    beclin-1 ELISA Kit, atg6 ELISA Kit, vps30 ELISA Kit, beclin1 ELISA Kit, Beclin-1 ELISA Kit, becn1 ELISA Kit, zgc:73097 ELISA Kit, ATG6 ELISA Kit, VPS30 ELISA Kit, 4921513J16Rik ELISA Kit, 5430417M23Rik ELISA Kit, Atg6 ELISA Kit, beclin 1 ELISA Kit, beclin 1, autophagy related ELISA Kit, beclin 1 protein ELISA Kit, beclin-1 ELISA Kit, beclin family protein ELISA Kit, beclin 1, autophagy related L homeolog ELISA Kit, BECN1 ELISA Kit, becn1 ELISA Kit, atg6 ELISA Kit, CpipJ_CPIJ011087 ELISA Kit, PTRG_07825 ELISA Kit, SJAG_01876 ELISA Kit, Becn1 ELISA Kit, becn1.L ELISA Kit
    Gene ID
    8678
    Pathways
    Autophagy
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