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Src ELISA Kit
Src
Reactivity: Human
pTyr419
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
Product Details Src ELISA Kit
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Target
See all Src ELISA Kits
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Binding Specificity
pTyr419
Reactivity
All reactivities for Src elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Human Phospho-SRC (Y419) Quantitative ELISA Kit. This assay quantitatively measures phosphorylated SRC (Tyr419) in lysate samples.
Sample Type
Cell Lysate, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes human SRC phosphorylated at site tyrosine 419.
Characteristics
Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Material not included
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Plate
Pre-coated
Protocol
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date
6 months
Target Details for Src
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Target
See all Src ELISA Kits
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Alternative Name
SRC (Src Products )
Synonyms
AW259666 ELISA Kit, pp60c-src ELISA Kit, C-src1 ELISA Kit, CG7524 ELISA Kit, D-Src64B ELISA Kit, D-src ELISA Kit, DSRC64 ELISA Kit, DSrc ELISA Kit, DSrc64 ELISA Kit, DSrc64B ELISA Kit, Dm SRC1 ELISA Kit, DmHD-358 ELISA Kit, Dmel\CG7524 ELISA Kit, Dsrc ELISA Kit, Dsrc64 ELISA Kit, Dsrc64B ELISA Kit, HD-358 ELISA Kit, MRE5 ELISA Kit, SRC 64B ELISA Kit, Src ELISA Kit, Src1 ELISA Kit, Src64 ELISA Kit, c-src ELISA Kit, dSrc ELISA Kit, dsrc ELISA Kit, src ELISA Kit, src1 ELISA Kit, src64 ELISA Kit, src64B ELISA Kit, src64b ELISA Kit, ASV ELISA Kit, SRC1 ELISA Kit, c-SRC ELISA Kit, p60-Src ELISA Kit, c-Src ELISA Kit, PP60C-SCR ELISA Kit, SDR ELISA Kit, SRC proto-oncogene, non-receptor tyrosine kinase ELISA Kit, uncharacterized LOC5574802 ELISA Kit, proto-oncogene tyrosine-protein kinase src ELISA Kit, Rous sarcoma oncogene ELISA Kit, Src oncogene at 64B ELISA Kit, SRC ELISA Kit, LOC5574802 ELISA Kit, CpipJ_CPIJ010659 ELISA Kit, Src ELISA Kit, Src64B ELISA Kit
Target Type
Viral Protein
Gene ID
6714
UniProt
P12931
Pathways
JAK-STAT Signaling , Neurotrophin Signaling Pathway , Intracellular Steroid Hormone Receptor Signaling Pathway , Regulation of Intracellular Steroid Hormone Receptor Signaling , Cellular Response to Molecule of Bacterial Origin , Cell-Cell Junction Organization , Regulation of Carbohydrate Metabolic Process , Autophagy , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Smooth Muscle Cell Migration , Negative Regulation of intrinsic apoptotic Signaling , Platelet-derived growth Factor Receptor Signaling , Thromboxane A2 Receptor Signaling , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling
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