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SARS-CoV-2 Spike ELISA Kit ELISA Kit

Reactivity: Human, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Sandwich ELISA Cell Culture Supernatant
Catalog No. ABIN6952757
$480.70
Plus shipping costs $45.00
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Delivery in 6 to 8 Business Days
  • Target
    Reactivity
    Human, SARS Coronavirus-2 (SARS-CoV-2)
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    SARS-CoV-2 (COVID-19) Spike Protein ELISA Kit. Detects the S-Protein (S2 subunit).
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Characteristics
    COVID-19 Spike Protein ELISA
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Target
    Gene ID
    43740568
    UniProt
    P0DTC2
  • Comment

    Standard protein: ABIN6952326

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Assay Procedure
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL standard or sample to each well. Incubate 2.5 hours at room temperature.
    3. Add 100 μL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
    4. Add 100 μL prepared Streptavidin solution. Incubate 45 minutes at room temperature.
    5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
    6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    12 months
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