SARS-CoV-2 N-Protein IgG Antibody ELISA Kit

Catalog No. ABIN6952759

Product Details

Target: SARS-CoV-2 N-Protein IgG Antibody

Reactivity: Human, SARS Coronavirus-2 (SARS-CoV-2)

Detection Method: Colorimetric

Method Type: Indirect ELISA

Application: ELISA

Purpose: COVID-19 (SARS-CoV-2) N-Protein Human Antibody Detection Kit (Indirect ELISA method) for the semi-quantitative measurement of human IgG antibody against SARS-CoV-2 in serum or plasma.

Sample Type: Plasma, Serum

Analytical Method: Semi-Quantitative

Characteristics: COVID-19 N-Protein Human IgG ELISA Kit

Components:

• SARS-CoV-2 N-protein coated 96-well microplate
• Wash Buffer
• Stop Solution
• Assay Diluent
• Positive Control
• HRP-anti positive control IgG Antibody
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol: This COVID19 human IgG antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 N protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for normalization purpose. The wells are washed and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 N protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Positive Control is from inactivated serum sample which contains SARS-COV-2 N protein human IgG antibody. We do not know the exact amounts of SARS-COV-2 N protein human IgG antibody in the Positive Control sample. The Positive Control only can be used as calibration curve for normalization purpose in different assays.

Assay Procedure:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
6. Read at 450 nm immediately.

Calculation of Results:

Calibration curve: Calculate the mean absorbance for each set of duplicate Positive Control (Item C), samples, and subtract the average zero Positive Control optical density. Plot the calibration curve on log-log with Positive Control concentration (Unit/mL) on the xaxis and absorbance on the y-axis using Sigma plot or Excel software.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 N-Protein IgG Antibody

Target Type: Antibody