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SARS-CoV-2 S1 Subunit (RBD) IgM Antibody ELISA Kit ELISA Kit

Reactivity: Human, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Indirect ELISA Blood
Catalog No. ABIN6952763
$475.20
Plus shipping costs $45.00
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  • Target
    Reactivity
    Human, SARS Coronavirus-2 (SARS-CoV-2)
    Detection Method
    Colorimetric
    Method Type
    Indirect ELISA
    Application
    ELISA
    Purpose
    COVID-19 S-Protein S1 Receptor Binding Domain Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgM antibody against SARS-CoV-2 in serum or dried blood.
    Sample Type
    Blood, Serum
    Analytical Method
    Quantitative
    Characteristics
    COVID-19 S-Protein (S1RBD) Human IgM Quantitative ELISA Kit
    Components
    • SARS-CoV-2 S-protein S1RBD coated 96-well microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent
    • Positive Control
    • HRP-anti positive control IgG Antibody
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Target
    Target Type
    Antibody
  • Plate
    Pre-coated
    Protocol
    This COVID19 human IgM antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 S1 RBD protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of AntibodyHRP-human IgM antibody-virus S1 RBD antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 S1 RBD IgM antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    Assay Procedure
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL positive control or sample to each well. Incubate 1.5 hours at room temperature.
    3. Add 100 μL prepared HRP- anti positive control IgG Antibody and/or 100 μL of prepared HRP-anti Human IgG Antibody to each well. Incubate 1 hour at room temperature.
    4. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
    5. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
    6. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    12 months
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