Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SARS-CoV-2 S-Protein IgG Antibody ELISA Kit ELISA Kit

Reactivity: Human, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Sandwich ELISA Serum
Catalog No. ABIN6952774
$761.54
Plus shipping costs $45.00
96 tests ABIN6952774
96 tests ABIN6952774
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    Reactivity
    Human, SARS Coronavirus-2 (SARS-CoV-2)
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    This SARS-CoV-2 IgG seroconversion assay is intended for the qualitative detection of SARS-CoV-2 specific antibodies of isotype IgG in plasma or serum.
    Sample Type
    Serum
    Analytical Method
    Qualitative
    Specificity
    SARS-CoV-2 specific antibodies of isotype IgG
    Characteristics
    This assay has been specifically developed using the SARS-CoV-2 RBD section of the spike protein. This delivers reliable and consistent results with a high degree of reproducibility.
    Components
    • 96-well antibody coated microtiter strip plate (removable wells 8x12) containing purified recombinant SARS-CoV-2 antigen, blocked and dried.
    • 10X Wash buffer 50ml
    • Diluent 22ml
    • Positive calibrator (lyophilized)
    • Negative calibrator (lyophilized)
    • Anti-human IgG horseradish peroxidase antibody
    • TMB substrate solution 10ml
    • Stop solution 5.5ml
    Material not included
    • Microtiter plate shaker capable of 300 rpm uniform horizontally circular movement
    • Manifold dispenser/aspirator or automated microplate washer
    • Microplate reader capable of measuring absorbance at 450 nm
    • Water bath or heat block capable of 56 °C incubation
    • Pipettes and Pipette tips
    • Deionized or distilled water
    • Polypropylene tubes for dilution of standard
    • Paper towels or laboratory wipes
  • Target
    Target Type
    Antibody, Antibody
  • Application Notes
    SARS-CoV-2 is the novel coronavirus that causes CoronaVirus Disease 2019 (COVID-19). Serological assays are critical for characterizing immune responses to viral infections by determining the presence of viral antigen specific antibodies in infected and recovered patient sera.
    Comment

    Controls: Normal human plasma or serum collected in the United States prior to November 2019 is an appropriate negative control. Plasma or purified immunoglobulin from COVID-19 convalescent patients or humanized mouse monoclonal antibodies could potentially serve as positive controls.

    Plate
    Pre-coated
    Protocol
    SARS-CoV-2 specific antibodies will bind to the purified recombinant HEK cell derived receptor-binding domain (RBD) of the SARS-CoV-2 spike protein coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal secondary anti-human IgG antibody binds to the captured protein. Excess secondary antibody is washed away and TMB substrate is used for color development at 450nm. Samples that exceed A450 values of 0.2 are designated positive by this assay.
    Reagent Preparation

    Wash buffer: Dilute 50 mL of 10X wash buffer concentrate with 450 mL of deionized water.

    Sample Preparation

    Heat inactivate plasma or serum samples in a 56 °C water bath or heat block for 1 hour as a general safety precaution. Samples are diluted 1:50 in diluent directly on the microtiter plate.

    Assay Procedure

    Perform assay at room temperature in a biological safety cabinet. Vigorously shake plate (300rpm) at each step of the assay.

    • Preparation of Calibrators: Reconstitute positive and negative calibrators by adding 100 μL of water directly to each vial and agitate gently to completely dissolve contents.
    • Sample Addition: Remove microtiter plate from bag and add 98 μL of diluent to the wells that will be used. Add 2 μL of calibrators and samples to wells in duplicate according to the plate layout. Carefully record position of samples. Shake plate at 300rpm for 30 minutes. Wash wells three times with 300 μL wash buffer. Remove excess wash by gently tapping plate on paper towel or kimwipe.
    • Antibody Addition: Briefly centrifuge vials before opening. Dilute 1 μL of HRP conjugated anti-human IgG into 10 mL diluent to generate a 1:5,000 dilution. Add 100 μL to all wells. Shake plate at 300rpm for 30 minutes. Wash wells three times with 300 μL wash buffer. Remove excess wash by gently tapping plate on paper towel or kimwipe.
    • Substrate Incubation: Add 100 μL TMB substrate to all wells and shake plate for 5 minutes. Substrate will change from colorless to different strengths of blue. Quench reaction by adding 50 μL of stop solution to all wells in the same order as substrate upon which color will change from blue to yellow. Mix thoroughly by gently shaking the plate.
    • Measurement: Set the absorbance at 450nm in a microtiter plate spectrophotometer. Measure the absorbance in all wells at 450nm. Subtract blank from all samples to determine corrected absorbance (A450).
    • Calculation of Results: Samples that exceed A450 values of 0.2 are designated positive by this assay.

    Restrictions
    For Research Use only
  • Storage
    4 °C,-80 °C
    Storage Comment
    Store all kit components at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted controls may be stored at -80°C for later use. Store all other unused kit components at 4°C. This kit should not be used beyond the expiration date.
You are here:
help Support