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SARS-CoV-2 S1 Subunit (RBD) IgG Antibody ELISA Kit ELISA Kit

Reactivity: Human, SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Indirect ELISA Serum
Catalog No. ABIN6952815
$804.76
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  • Target
    Reactivity
    Human, SARS Coronavirus-2 (SARS-CoV-2)
    Detection Method
    Colorimetric
    Method Type
    Indirect ELISA
    Application
    ELISA
    Purpose
    COVID-19 S-Protein S1 Receptor Binding Domain Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum.
    Sample Type
    Serum
    Analytical Method
    Quantitative
    Characteristics
    COVID-19 S-Protein (S1RBD) Human IgG ELISA Kit
    Components
    • SARS-CoV-2 S-protein S1RBD coated 96-well microplate
    • Wash Buffer
    • Positive Control
    • Negative Control
    • Biotinylated Anti-Human IgG
    • HRP-Streptavidin concentrate
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diluent
    • 5X Sample Diluent
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plate
    Pre-coated
    Protocol
    This COVID19 human IgG antibody ELISA kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 S protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for normalization purpose. The wells are washed and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 S protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
    Assay Procedure
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
    3. Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
    4. Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
    5. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
    6. Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    12 months
  • Target
    Target Type
    Antibody
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