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Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay kit Kit

ATA Reactivity: SARS Coronavirus-2 (SARS-CoV-2) Colorimetric Plasma Without plate
Catalog No. ABIN6953179
$578.00
Plus shipping costs $45.00
96 tests
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Will be delivered in 5 Business Days
  • Target
    Reactivity
    SARS Coronavirus-2 (SARS-CoV-2)
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Application
    Antibody Titer Assay (ATA)
    Purpose
    This assay kit employs a competitive ELISA format and uniquely suitable for rapid high-throughput detecting the titer of Anti-SARS-CoV-2 neutralizing antibody in serum / plasma.
    Sample Type
    Plasma, Serum
    Components
    • SARS-CoV-2 S protein RBD
    • Biotinylated Human ACE2
    • Streptavidin-HRP
    • Anti-SARS-CoV-2 neutralizing antibody
    Material not included
    ELISA Buffers and plate are sold separately and not included in kit.
  • Application Notes
    This kit is useful for serologic test for the titer of Anti-SARS-CoV-2 neutralizing antibody in serum/plasma in vitro.
    Plate
    Without plate
    Protocol
    Briefly, we provide you with a biotinylated Human ACE2 protein, a SARS-CoV-2 S protein RBD protein, an Anti-SARS-CoV-2 neutralizing antibody (human IgG1) (as method verified Reference), and Streptavidin-HRP reagent.
    Your experiment will include 4 simple steps:
    1. Coat the plate with SARS-CoV-2 S protein RBD.
    2. Add 50 µL biotinylated human ACE2 to coated plate.
    3. Add the reference in kit or your samples in serum or plasma of interest.
    4. Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
    Finally, the titer of your samples to inhibit S protein RBD: ACE2 binding will be determined by comparing OD readings among different experimental groups.
    Restrictions
    For Research Use only
  • Handling Advice
    Please avoid repeated freeze-thaw cycles.
    Storage
    RT,-20 °C,-80 °C
    Storage Comment
    Upon receipt, please store all items at -70°C.After reconstitution, the stock solution should be kept at -70°C.
    It is recommended not to freeze thaw more than 3 times.
    No activity loss is observed after storage at:
    • Room temperature (RT) for 1 month in lyophilized state
    • -20°C for 1 year in lyophilized state
    • -70°C for 6 months under sterile conditions after reconstitution.

  • Target Name (Antigen)
    Alternative Name
    SARS-Coronavirus-2
    Target Type
    Virus
    Background
    The newly identified 2019 novel coronavirus (SARS-CoV-2) has posed a serious threat to human health, and it spreads quickly. It is helpful to develop the Anti SARS-CoV-2 neutralizing antibody titer serologic assay kit to test the protective neutralizing antibody level in serum.
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