SARS-CoV-2 IgG Antibody Multiplex ELISA Kit
mpELISA
Reactivity: Human
Qualitative
Plasma
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Catalog No. ABIN6953214
$1,344.00
Plus shipping costs $45.00
96 tests
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Shipping to:
United States
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- Key Features
- Multiplex ELISA: Testing 5 Targets in 1 Run
- High performance COVID-19 antibody testing
- 99.6% specificity, 95.9% sensitivity
- Target
- SARS-CoV-2 IgG Antibody
- Reactivity
- Human
- Application
- Multiplex ELISA (mpELISA)
- Purpose
- Multiplex kit for COVID-19 serological testing and immune status characterization.
- Sample Type
- Plasma
- Analytical Method
- Qualitative
- Specificity
- 5 antigens printed side by side in the same well: Nucleocapsid (N), Spike 1 subunit (S1), receptor binding domain of the S1 (S1-RBD), N-terminal domain of the S1 (S1-NTD), and Spike 2 subunit (S2).
The combination of N and S1 antigens increases diagnostic performances: 99.6 % specificity and 95.9 % sensitivity. The addition of the S1-RBD, S1-NTD and S2 antigens enlarge again the comprehensive picture of the humoral response diversity. - Characteristics
- The kit allows high performance COVID-19 antibody testing. It detects the presence of IgG against SARS-Cov2 in patient sera and indicates whether these antibodies are targeting the S- and/or the N-protein of SARS-CoV-2. All in one sample. The combined detection of Nucleocapsid and Spike 1 antibodies increases the chance to detect the immune response, especially under 14 days after symptoms onset. The multiplex approach enables user to get a more comprehensive picture of the patient immune profile in a single assay: it brings a high added value to understand how the patient is protected.
- Components
- Precoated plate
- Diluent Buffer
- Wash Buffer
- Conjugate
- Substrate
- Material not included
- Precision micropipettes with suitable tips
- Distilled or deionized water
- Microplate washer (recommended)
- Microplate shaker & incubator (recommended)
- Colorimetric biochips reader (optional)
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- Application Notes
- powerful and easy to use technology
- processes 4 to 200 tests
- minimized biological sample volume
- 2-hour turnaround time
- standard ELISA materials
- Comment
Multiplex ELISA-like test for the serodetection of different anti-SARS-CoV-2 IgG antibodies. The kit is dedicated to IgG detection (IgM and IgA can be detected if needed).
- Assay Time
- 2 - 3 h
- Protocol
- Dilute samples at 1:100 in diluent buffer
- Incubate plates at 37 °C for 1 h on a microplate shaker at 300 rpm
- Wash 3 times with wash buffer
- Dilute conjugate at 6:1000 in diluent buffer
- Add Conjugate to plate
- Incubate at 37 °C for 1 h
- Wash 3 times with wash buffer
- Add substrate to plate
- Incubate at room temperature for 15 min
- Rinse wells and dry 15 min at 37 °C
- Picture acquisition
- Calculation of Results
Visual interpretation: check for the presence of the "N" and/or "S" letters at the bottom of the well to identify the sample as positive.
In order to confirm visual checking, pictures can be acquired with a dedicated reader and analyzed using a software and interpretation algorithm.- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target
- SARS-CoV-2 IgG Antibody
- Target Type
- Antibody
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