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SARS-CoV-2 IgG Antibody Multiplex ELISA Kit

mpELISA Reactivity: Human Qualitative Plasma
Catalog No. ABIN6953214
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 7 to 8 Business Days
  • Key Features
    • Multiplex ELISA: Testing 5 Targets in 1 Run
    • High performance COVID-19 antibody testing
    • 99.6% specificity, 95.9% sensitivity
    SARS-CoV-2 IgG Antibody
    Multiplex ELISA (mpELISA)
    Multiplex kit for COVID-19 serological testing and immune status characterization.
    Sample Type
    Analytical Method
    5 antigens printed side by side in the same well: Nucleocapsid (N), Spike 1 subunit (S1), receptor binding domain of the S1 (S1-RBD), N-terminal domain of the S1 (S1-NTD), and Spike 2 subunit (S2).
    The combination of N and S1 antigens increases diagnostic performances: 99.6 % specificity and 95.9 % sensitivity. The addition of the S1-RBD, S1-NTD and S2 antigens enlarge again the comprehensive picture of the humoral response diversity.
    The kit allows high performance COVID-19 antibody testing. It detects the presence of IgG against SARS-Cov2 in patient sera and indicates whether these antibodies are targeting the S- and/or the N-protein of SARS-CoV-2. All in one sample. The combined detection of Nucleocapsid and Spike 1 antibodies increases the chance to detect the immune response, especially under 14 days after symptoms onset. The multiplex approach enables user to get a more comprehensive picture of the patient immune profile in a single assay: it brings a high added value to understand how the patient is protected.
    • Precoated plate
    • Diluent Buffer
    • Wash Buffer
    • Conjugate
    • Substrate
    Material not included
    • Precision micropipettes with suitable tips
    • Distilled or deionized water
    • Microplate washer (recommended)
    • Microplate shaker & incubator (recommended)
    • Colorimetric biochips reader (optional)
  • Application Notes
    • powerful and easy to use technology
    • processes 4 to 200 tests
    • minimized biological sample volume
    • 2-hour turnaround time
    • standard ELISA materials

    Multiplex ELISA-like test for the serodetection of different anti-SARS-CoV-2 IgG antibodies. The kit is dedicated to IgG detection (IgM and IgA can be detected if needed).

    Assay Time
    2 - 3 h
    • Dilute samples at 1:100 in diluent buffer
    • Incubate plates at 37 °C for 1 h on a microplate shaker at 300 rpm
    • Wash 3 times with wash buffer
    • Dilute conjugate at 6:1000 in diluent buffer
    • Add Conjugate to plate
    • Incubate at 37 °C for 1 h
    • Wash 3 times with wash buffer
    • Add substrate to plate
    • Incubate at room temperature for 15 min
    • Rinse wells and dry 15 min at 37 °C
    • Picture acquisition
    Calculation of Results

    Visual interpretation: check for the presence of the "N" and/or "S" letters at the bottom of the well to identify the sample as positive.
    In order to confirm visual checking, pictures can be acquired with a dedicated reader and analyzed using a software and interpretation algorithm.

    For Research Use only
  • Storage
    4 °C
  • Target
    SARS-CoV-2 IgG Antibody
    Target Type
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