SARS-CoV-2 IgG Antibody Multiplex ELISA

Catalog No. ABIN6953214

Product Details

Key Features:

• Multiplex ELISA: Testing 5 Targets in 1 Run
• High performance COVID-19 antibody testing
• 99.6% specificity, 95.9% sensitivity

Target:

SARS-CoV-2 IgG Antibody

Reactivity: Human

Application: Multiplex ELISA (mpELISA)

Purpose: Multiplex kit for COVID-19 serological testing and immune status characterization.

Sample Type: Plasma

Analytical Method: Qualitative

Specificity: 5 antigens printed side by side in the same well: Nucleocapsid (N), Spike 1 subunit (S1), receptor binding domain of the S1 (S1-RBD), N-terminal domain of the S1 (S1-NTD), and Spike 2 subunit (S2).
The combination of N and S1 antigens increases diagnostic performances: 99.6 % specificity and 95.9 % sensitivity. The addition of the S1-RBD, S1-NTD and S2 antigens enlarge again the comprehensive picture of the humoral response diversity.

Characteristics: The kit allows high performance COVID-19 antibody testing. It detects the presence of IgG against SARS-Cov2 in patient sera and indicates whether these antibodies are targeting the S- and/or the N-protein of SARS-CoV-2. All in one sample. The combined detection of Nucleocapsid and Spike 1 antibodies increases the chance to detect the immune response, especially under 14 days after symptoms onset. The multiplex approach enables user to get a more comprehensive picture of the patient immune profile in a single assay: it brings a high added value to understand how the patient is protected.

Components:

• Precoated plate
• Diluent Buffer
• Wash Buffer
• Conjugate
• Substrate

Material not included:

• Precision micropipettes with suitable tips
• Distilled or deionized water
• Microplate washer (recommended)
• Microplate shaker & incubator (recommended)
• Colorimetric biochips reader (optional)

Target Details

Target Name (Antigen):

SARS-CoV-2 IgG Antibody

Target Type: Antibody

Application Details

Application Notes:

• powerful and easy to use technology
• processes 4 to 200 tests
• minimized biological sample volume
• 2-hour turnaround time
• standard ELISA materials

Comment:

Multiplex ELISA-like test for the serodetection of different anti-SARS-CoV-2 IgG antibodies. The kit is dedicated to IgG detection (IgM and IgA can be detected if needed).

Assay Time: 2 - 3 h

Protocol:

• Dilute samples at 1:100 in diluent buffer
• Incubate plates at 37 °C for 1 h on a microplate shaker at 300 rpm
• Wash 3 times with wash buffer
• Dilute conjugate at 6:1000 in diluent buffer
• Add Conjugate to plate
• Incubate at 37 °C for 1 h
• Wash 3 times with wash buffer
• Add substrate to plate
• Incubate at room temperature for 15 min
• Rinse wells and dry 15 min at 37 °C
• Picture acquisition

Calculation of Results:

Visual interpretation: check for the presence of the "N" and/or "S" letters at the bottom of the well to identify the sample as positive.
In order to confirm visual checking, pictures can be acquired with a dedicated reader and analyzed using a software and interpretation algorithm.

Restrictions: For Research Use only

Handling

Storage: 4 °C