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HSP90 ELISA Kit

HSP90 Reactivity: Mouse Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN6963614
  • Target See all HSP90 ELISA Kits
    HSP90 (Heat Shock Protein 90 (HSP90))
    Reactivity
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.16 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.16 ng/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This kit recognizes Mouse HSP-90 in samples. No Significant cross-reactivity or interference between Mouse HSP-90 and analogues was observed.
    Sensitivity
    0.1 ng/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Reference Standard & Sample Diluent
    • Biotinylated Detection Antibody (100 x concentrate)
    • HRP Conjugate (100 x concentrate)
    • Biotinylated Detection Antibody Diluent
    • HRP Conjugate Diluent
    • Substrate Reagent
    • Stop Solution
    • Wash Buffer (25 x concentrate)
    • Instruction manual
    Top Product
    Discover our top product HSP90 ELISA Kit
  • Sample Volume
    100 μL
    Assay Time
    3.5 h
    Plate
    Pre-coated
    Protocol
    1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
    2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
    3. Aspirate and wash 3 times.
    4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
    5. Aspirate and wash 5 times.
    6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
    7. Add 50 µL Stop Solution. Read at 450 nm immediately.
    8. Calculation of results.
    Reagent Preparation
    1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
    2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
    3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 10 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 10, 5, 2.5, 1.25, 0.63, 0.31, 0.16, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 10 ng/mL working solution to the first tube and mix up to produce a 5 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
    4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
    5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
    Sample Preparation
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HSP-90 were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HSP-90 were tested on 3 different plates, 20 replicates in each plate.
    Both intra-CV and inter-CV are < 10 %.
    Restrictions
    For Research Use only
  • Storage
    4 °C,-20 °C
    Storage Comment
    1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 6 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
    2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
    Expiry Date
    6 months
  • Target See all HSP90 ELISA Kits
    HSP90 (Heat Shock Protein 90 (HSP90))
    Alternative Name
    Heat Shock Protein 90 (HSP90 Products)
    Synonyms
    EL52 ELISA Kit, HSP86 ELISA Kit, HSP89A ELISA Kit, HSP90A ELISA Kit, HSP90N ELISA Kit, HSPC1 ELISA Kit, HSPCA ELISA Kit, HSPCAL1 ELISA Kit, HSPCAL4 ELISA Kit, HSPN ELISA Kit, Hsp89 ELISA Kit, Hsp90 ELISA Kit, LAP2 ELISA Kit, git10 ELISA Kit, swo1 ELISA Kit, HSP90 ELISA Kit, htpG ELISA Kit, SCBAC25F8.08 ELISA Kit, 23.m06066 ELISA Kit, 17.m07646 ELISA Kit, HSP90-1 ELISA Kit, 143198_at ELISA Kit, 83 ELISA Kit, 83K HSP ELISA Kit, DMHSP82 ELISA Kit, E(sev)3A ELISA Kit, E(sina)2 ELISA Kit, HSP82 ELISA Kit, HSP83 ELISA Kit, ORF1 ELISA Kit, Su(Raf)3A ELISA Kit, anon-EST:Liang-2.53 ELISA Kit, anon-WO0068693 ELISA Kit, anon-WO0140519.209 ELISA Kit, clone 2.53 ELISA Kit, en(lz)3C/4C ELISA Kit, hsp84 ELISA Kit, l(3)j5C2 ELISA Kit, ms(3)08445 ELISA Kit, stc ELISA Kit, DmelCG1242 ELISA Kit, CG1242 ELISA Kit, Hsp86 ELISA Kit, Hspca ELISA Kit, 86kDa ELISA Kit, 89kDa ELISA Kit, AL024080 ELISA Kit, AL024147 ELISA Kit, Hsp86-1 ELISA Kit, hsp4 ELISA Kit, hsp86 ELISA Kit, hsp89 ELISA Kit, hsp90 ELISA Kit, hsp90a ELISA Kit, hspc1 ELISA Kit, hspca ELISA Kit, hspn ELISA Kit, lap2 ELISA Kit, Hsp90alpha ELISA Kit, heat shock protein 90 alpha family class A member 1 ELISA Kit, heat shock protein Hsp90 ELISA Kit, Hsp90 chaperone ELISA Kit, Heat shock protein 90 ELISA Kit, heat shock protein 90 ELISA Kit, chaperone protein HtpG ELISA Kit, molecular chaperone HtpG ELISA Kit, Heat Shock Protein 90 ELISA Kit, uncharacterized LOC100384473 ELISA Kit, Heat shock protein 83 ELISA Kit, heat shock protein 90, alpha (cytosolic), class A member 1 ELISA Kit, heat shock protein 90 alpha family class B member 1 ELISA Kit, heat shock protein 90kDa alpha family class A member 1 L homeolog ELISA Kit, heat shock protein HSP 90-alpha ELISA Kit, HSP90AA1 ELISA Kit, hsp90 ELISA Kit, HSP90 ELISA Kit, daf-21 ELISA Kit, htpG ELISA Kit, SCO7516 ELISA Kit, MAP_RS10510 ELISA Kit, TP04_0646 ELISA Kit, TP01_0934 ELISA Kit, APH_RS03525 ELISA Kit, GbCGDNIH1_0315 ELISA Kit, MAV_2118 ELISA Kit, HSP90C ELISA Kit, BBOV_IV008400 ELISA Kit, BBOV_III007380 ELISA Kit, ACICU_00312 ELISA Kit, ECL_01244 ELISA Kit, YE105_C1172 ELISA Kit, pco153543(105) ELISA Kit, Hsp83 ELISA Kit, Hsp90aa1 ELISA Kit, HSP90AB1 ELISA Kit, hsp90aa1.1.L ELISA Kit, LOC108698781 ELISA Kit
    Background
    Hsp90
    Pathways
    M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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