TGFA ELISA Kit

Catalog No. ABIN921075

Product Details TGFA ELISA Kit

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Binding Specificity: AA 40-89

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TGF alpha

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli,V40-A89

Cross-Reactivity (Details): There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1 % .

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: V40-A89

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. .

Plate: Pre-coated

Protocol: human TGF alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF alpha has been precoated onto 96-well plates. Standards (E.coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGF alpha amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGF alpha standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 118, Standard deviation: 5.43, CV(%): 4.6
• Sample 2: n=16, Mean(pg/ml): 225, Standard deviation: 12.83, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 516, Standard deviation: 16.5, CV(%): 3.2,
• Sample 1: n=24, Mean(pg/ml): 126, Standard deviation: 8.95, CV(%): 7.1
• Sample 2: n=24, Mean(pg/ml): 238, Standard deviation: 14.28, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 509, Standard deviation: 27, CV(%): 5.3

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for TGFA ELISA Kit (ABIN921075)

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, (2015) (PubMed).

Qiu, Zhu, Lu, Bai, Liu, Zhao, Sun, Zhang, Li, Liu: "The selective mGluR5 agonist CHPG attenuates SO2-induced oxidative stress and inflammation through TSG-6/NF-?B pathway in BV2 microglial cells." in: Neurochemistry international, Vol. 85-86, pp. 46-52, (2015) (PubMed).

Chen, Qi, Feng, Wang, Bao, Wang, Xiang, Xie: "Neuroprotective effect of allicin against traumatic brain injury via Akt/endothelial nitric oxide synthase pathway-mediated anti-inflammatory and anti-oxidative activities." in: Neurochemistry international, Vol. 68, pp. 28-37, (2014) (PubMed).

Yu, Liu, Chen, Zeng: "Anti-inflammatory effect of Qingwen Baidu Decoction (?????) in sepsis rats." in: Chinese journal of integrative medicine, Vol. 20, Issue 12, pp. 934-43, (2014) (PubMed).

Gao, Huang, Li: "NOX1 abet mesangial fibrogenesis via iNOS induction in diabetes." in: Molecular and cellular biochemistry, Vol. 382, Issue 1-2, pp. 185-91, (2013) (PubMed).

Huang, Zhang, Wang, Yu, Fang, Lu, Zhang, Wei: "Transforming growth factor ?1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1." in: Journal of neuroinflammation, Vol. 9, pp. 145, (2012) (PubMed).

Mao, Gao, Li, Li: "Pigment epithelium-derived factor inhibits high glucose induced oxidative stress and fibrosis of cultured human glomerular mesangial cells." in: Saudi medical journal, Vol. 32, Issue 8, pp. 769-77, (2011) (PubMed).

Ye, Wu, Guo, Wang, Yuan, Shang, Yao: "Lipoxin A4 analogue protects brain and reduces inflammation in a rat model of focal cerebral ischemia reperfusion." in: Brain research, Vol. 1323, pp. 174-83, (2010) (PubMed).

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGFA (TGFA Products)

Synonyms: TFGA ELISA Kit, LOC100009482 ELISA Kit, TGF alpha ELISA Kit, TGFA ELISA Kit, wa-1 ELISA Kit, wa1 ELISA Kit, RATTGFAA ELISA Kit, TGFAA ELISA Kit, NV15606 ELISA Kit, si:ch211-10e8.7 ELISA Kit, transforming growth factor alpha ELISA Kit, transforming growth factor alpha L homeolog ELISA Kit, transforming growth factor-alpha ELISA Kit, transforming growth factor, alpha ELISA Kit, TGFA ELISA Kit, TGF-A ELISA Kit, Tgfa ELISA Kit, tgfa.L ELISA Kit, LOC100121300 ELISA Kit, LOC100009150 ELISA Kit, tgfa ELISA Kit

Background:

Protein Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Synonyms: Protransforming growth factor alpha,Transforming growth factor alpha,TGF-alpha,EGF-like TGF,ETGF,TGF type 1,TGFA,

Full Gene Name: Protransforming growth factor alpha

Cellular Localisation: Transforming growth factor alpha: Secreted, extracellular space.

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway