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TNFRSF11A ELISA Kit

TNFRSF11A Reactivity: Human AA 29-213 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Serum
Catalog No. ABIN921105
  • Target See all TNFRSF11A ELISA Kits
    TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
    Binding Specificity
    AA 29-213
    Reactivity
    • 4
    • 3
    • 3
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human RANK
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: Q29-G213
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <2pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: Q29-G213
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Sequence similarities: Contains 4 TNFR-Cys repeats.

    Tissue Specificity: Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.

    Plate
    Pre-coated
    Protocol
    human RANK ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for RANK has been precoated onto 96-well plates. Standards(NSO,Q29-G213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RANK is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human RANK amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human RANK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human RANK standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 655, Standard deviation: 26.2, CV(%): 4
    • Sample 2: n=16, Mean(pg/ml): 1863, Standard deviation: 68.93, CV(%): 3.7
    • Sample 3: n=16, Mean(pg/ml): 2533, Standard deviation: 136.8, CV(%): 5.4,
    • Sample 1: n=24, Mean(pg/ml): 639, Standard deviation: 37.1, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 1957, Standard deviation: 90, CV(%): 4.6
    • Sample 3: n=24, Mean(pg/ml): 2668, Standard deviation: 176.1, CV(%): 6.6
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all TNFRSF11A ELISA Kits
    TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))
    Alternative Name
    RANK (TNFRSF11A Products)
    Synonyms
    RANK ELISA Kit, RGD1563614 ELISA Kit, TNFRSF11A ELISA Kit, Ly109 ELISA Kit, ODFR ELISA Kit, OFE ELISA Kit, Rank ELISA Kit, TRANCE-R ELISA Kit, mRANK ELISA Kit, CD265 ELISA Kit, FEO ELISA Kit, LOH18CR1 ELISA Kit, OPTB7 ELISA Kit, OSTS ELISA Kit, PDB2 ELISA Kit, TRANCER ELISA Kit, TNF receptor superfamily member 11A ELISA Kit, TNF receptor superfamily member 11a ELISA Kit, tumor necrosis factor receptor superfamily, member 11a, NFKB activator ELISA Kit, Tnfrsf11a ELISA Kit, TNFRSF11A ELISA Kit
    Background

    Protein Function: Receptor for TNFSF11/RANKL/TRANCE/OPGL, essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells. .

    Background: Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.

    Synonyms: Tumor necrosis factor receptor superfamily member 11A,Osteoclast differentiation factor receptor,ODFR,Receptor activator of NF-KB,CD265,TNFRSF11A,RANK,

    Full Gene Name: Tumor necrosis factor receptor superfamily member 11A

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    8792
    UniProt
    Q9Y6Q6
    Pathways
    NF-kappaB Signaling
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