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Ghrelin ELISA Kit

GHRL Reactivity: Human Colorimetric Competition ELISA 123.5-10000 pg/mL
Catalog No. ABIN955873
  • Target See all Ghrelin (GHRL) ELISA Kits
    Ghrelin (GHRL)
    Reactivity
    • 5
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    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
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    Human
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    123.5-10000 pg/mL
    Minimum Detection Limit
    123.5 pg/mL
    Application
    ELISA
    Purpose
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of human GHRL in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of human GHRL. No significant cross-reactivity or interference between human GHRL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human GHRL and all the analogues, therefore, cross reaction may still exist.
    Sensitivity
    123.5 - 10000 pg/mL
    The minimum detectable dose of human GHRL is typically less than 45.3 pg/mL.
    The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations.
    Characteristics
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for human GHRL has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled human GHRL and unlabeled human GHRL (Calibrators or samples) with the pre-coated antibody specific for human GHRL. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GHRL in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GHRL in the sample.
    Components
    Pre-coated, ready to use 96-well strip plate (1x)
    Calibrator (lyophilized) (2x)
    Calibrator Diluent (1 x 20 mL)
    Detection Reagent A (1 x 120 µL)
    Detection Reagent B (1 x 120 µL)
    Assay Diluent A (2X concentrate) (1 x 6 mL)
    Assay Diluent B (2X concentrate) (1 x 6 mL)
    TMB Substrate (1 x 9 mL)
    Stop Solution (1 x 6 mL)
    Wash Buffer (30X concentrate) (1 x 20 mL)
    Plate sealer for 96 wells (4x).
    Material not included
    1. Microplate reader with 450 +/- 10 nm filter.
    2. Precision single and multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. De-ionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Comment

    The calibration curve concentrations used for the ELISA's were 10, 000 pg/mL, 3, 333.3 pg/mL, 1111.1 pg/mL, 370.4 pg/mL, 123.5 pg/mL.

    Plate
    Pre-coated
    Calculation of Results

    This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between GHRL concentration in the sample and the assay signal intensity. Average the duplicate readings for each calibrator, control, and samples. Create a calibration curve on log-log or semi-log graph paper, with GHRL concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
    Expiry Date
    The expiry date is stated on the label.
  • Target See all Ghrelin (GHRL) ELISA Kits
    Ghrelin (GHRL)
    Alternative Name
    Ghrelin (GHRL Products)
    Synonyms
    2210006E23Rik ELISA Kit, Ghr ELISA Kit, MTLRP ELISA Kit, MTLRPAP ELISA Kit, m46 ELISA Kit, Gh1-1 ELISA Kit, GRHL ELISA Kit, MTLRP-AP ELISA Kit, GHR ELISA Kit, ghrelin ELISA Kit, ghrelin and obestatin prepropeptide ELISA Kit, ghrelin/obestatin prepropeptide ELISA Kit, Ghrl ELISA Kit, GHRL ELISA Kit, ghrl ELISA Kit
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Negative Regulation of Hormone Secretion, Synaptic Membrane, Feeding Behaviour
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