Luminescent immunoassays are variations of the standard ELISA, just like fluorescent immunoassays. The Chemiluminescent Immunoassay kit (CLIA kit) is designed for the in vitro sensitive quantitative measurement of plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. An enzyme converts a substrate to a reaction product that emits photons of light instead of developing a visible color.
Luminescence is described as the emission of light from a substance as it returns from an electronically excited state to ground state. The chemiluminescent substance is excited by the oxidation and catalysis forming intermediates. When the excited intermediates return back to their stable ground state, a photon is released, which is detected by the luminescent signal instrument.
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