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TR-FRET Assays

TR-FRET (Time-Resolved Fluorescence Energy Transfer) is a special form of FRET (Förster Resonance Energy Transfer) in which the long-lived emission fluorophores are used as donors. Sandwich TR-FRET immunoassays for semi-quantitative detection enable the examination of phosphorylation events in cellular signaling pathways. We offer high quality TR-FRET Assay Kit alternatives at an competitive price. Our kits come in convenient sizes of 100 and 500 points for 69 key targets.

TR-FRET Method and Products - antibodies-online.com

Advantages of TR-FRET Assays

TR-FRET Asasays are a truly homogeneous assay technology platform. The technique combines the robust FRET approach with the sensitivity of TRF and is often utilized in high throughput screening and drug discovery. However the assay is also gaining popularity in basic research. In comparison with ELISA and WB, TR-FRET offers distinct advantages:

  • No washing steps
  • Single incubation step
  • Save hands on time
  • Increase throughput
  • Increase reproducibility

Phospho-Protein TR-FRET Assays

Our Phospho-Protein Kits enable the detection of proteins phosphorylated at defined residues. Total-Protein Kits allow the detection of total protein in a sample and can be used to normalize your data obtained with the Phospho-Kits. The Phospho- and Total Protein Kits are designed for simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit. The assays are compatible with both adherent and suspension cells, and are conducted in just 3 steps. Cells are first treated to modulate protein levels. Cells are then lysed with the specific lysis buffer provided in the kit and the target protein is subsequently detected in one-step using the TR-FRET reagents. The Phospho- and Total Protein Kits are designed for simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit.

  • Simultaneous measurement of two proteins in separate wells from the same cell lysate using a single kit.
  • 3 steps only
  • Compatible with both adherent and suspension cells
  • Positive control in both kit types included

New Cytokine TR-FRET Assays Available

antibodies-online now offers assays for reliable detection of cytokines. The simple, rapid and sensitive immunoassay is based on the homogeneous (no-wash) TR-FRET technology. The assays are easy to perform with only 4 steps and 1 h incubation in total. A distinct advantage of the TR-FRET technology is that it does not require addition of potassium fluoride to ensure signal stability. The non-toxic nature of makes it an ideal candidate for multiplexing with other assay technologies using a sequential assay protocol.

Product
Sample Type
Analytical Method
Detection Range
Cat. No.
Quantity
Sample TypeCell Culture Supernatant
Analytical MethodQuantitative
Detection Range21 pg/mL - 30000 pg/mL
Cat. No.ABIN6999323
Quantity100 points
Sample TypeCell Culture Supernatant
Analytical MethodQuantitative
Detection Range26 pg/mL - 10000 pg/mL
Cat. No.ABIN6999324
Quantity100 points
Sample TypeCell Culture Supernatant
Analytical MethodQuantitative
Detection Range10 pg/mL - 100000 pg/mL
Cat. No.ABIN6999325
Quantity100 points
Sample TypeCell Culture Supernatant
Analytical MethodQuantitative
Detection Range35 pg/mL - 30000 pg/mL
Cat. No.ABIN6999326
Quantity100 points
Sample TypeCell Culture Supernatant
Analytical MethodQuantitative
Detection Range15 pg/mL - 10000 pg/mL
Cat. No.ABIN6999327
Quantity100 points
Sample TypeCell Culture Supernatant
Analytical MethodQuantitative
Detection Range13 pg/mL - 30000 pg/mL
Cat. No.ABIN6999322
Quantity100 points

Highly validated components and Data-transparency

Comprehensive Technical Data Sheets showing the pharmacological validation of each assay kit are available. Browse our available TR-FRET assays down below! Our support team is happy to help you select the proper kit for your research. We can answer any questions you may have. Please contact us via phone, email or use our live chat system.

74 results:

Phospho-4EBP1 (T37/T46) and Total 4EBP1 TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat, Monkey Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938945
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-CREB (S133) and Total CREB TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938956
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-STAT6 (Y641) and Total STAT6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938969
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-Ribosomal Protein S6 (S240/244) and Total Ribosomal Protein S6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938973
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-Ribosomal Protein S6 (S235/236) and Total Ribosomal Protein S6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938975
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-STAT5 (Y694/Y699) and Total STAT5 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938990
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-Met (Y1234/1235) and Total MET TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6939009
$1,157.70 $1,362.00 Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-4EBP1 (T37/T46) TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat, Monkey Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938946
$341.70 $402.00 Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-BAD (S112) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat, Monkey Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938981
$341.70 $402.00 Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-CREB (S133) TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938957
$341.70 $402.00 Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
  • Type Kit
    • Kit
  • Target
    • Ribosomal Protein S6 (RPS6)
    • V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
    • Epidermal Growth Factor Receptor (EGFR)
    • Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A)
    • Eukaryotic Translation Initiation Factor 4E (EIF4E)
    • Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)
    • Glycogen Synthase Kinase 3 beta (GSK3b)
    • Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)
    • Met Proto-Oncogene (MET)
    • Mitogen-Activated Protein Kinase 1/3 (MAPK1/3)
    • Mitogen-Activated Protein Kinase 8 (MAPK8)
    • Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
    • Nuclear Factor kappa B (NFkB)
    • Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)
    • STAT5 A/B
    • Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
    • Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6)
    • Tumor Protein P53 (TP53)
    • cAMP Responsive Element Binding Protein 1 (CREB1)
    • BCL2-Associated Agonist of Cell Death (BAD)
    • Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
    • Insulin Receptor beta (ISR-beta)
    • Interferon gamma (IFNG)
    • Interleukin 1, beta (IL1B)
    • Interleukin 2 (IL2)
    • Interleukin 6 (IL6)
    • Interleukin 8 (IL8)
    • Mitogen-Activated Protein Kinase 14 (MAPK14)
    • Proto-oncogene tyrosine-protein kinase Src (Src)
    • SMAD, Mothers Against DPP Homolog 2 (SMAD2)
    • Tumor Necrosis Factor alpha (TNF)
    • p38 alpha beta gamma
  • Application Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • ELISA
    • Biochemical Assay (BCA)
    • Functional Studies (Func)
    • Activity Assay (AcA)
    • Detection (D)
    • Quantification (Q)
    • Western Blotting (WB)
    • Enzyme Activity Assay (EAA)
    • Labeling (Lbl)
    • Cellular Assay (CA)
    • Screening Assay (ScA)
    • Flow Cytometry (FACS)
    • ELISpot
    • Fluorescence Resonance Energy Transfer Microscopy (FRET)
    • Purification (Purif)
    • Conjugation (Con)
    • Binding Studies (Bind)
    • Immunoprecipitation (IP)
    • Chromatin Immunoprecipitation (ChIP)
    • Immunofluorescence (IF)
    • Apoptosis Detection (AD)
    • Immunohistochemistry (IHC)
    • Mass Spectrometry (MS)
    • Inhibition Assay (InhA)
    • Colloidal Gold Immunochromatography Assay (GICA)
    • Immunocytochemistry (ICC)
    • Protein Complex Immunoprecipitation (Co-IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • High Pressure Liquid Chromatography (HPLC)
    • Pull-Down Assay (Pull-Down)
    • Affinity Measurement (AM)
    • Fluorescence Microscopy (FM)
    • Activation (Act)
    • Immunoassay (IA)
    • Rapid Test (rapid)
    • Cell Viability Assay (CVA)
    • High-throughput Screening (HTS)
    • Cytotoxicity Test (CyTox)
    • Proliferation Assay (ProA)
    • Isotyping (IsoT)
    • Staining Methods (StM)
    • Separation (Sep)
    • Cell Culture (CC)
    • Light Microscopy (LM)
    • Sample Preparation (Prep)
    • SDS-PAGE (SDS)
    • Protein Extraction (PEx)
    • Substrate (S)
    Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Monkey
    • Cow
    • Dog
    • Hamster
    • Pig
    • Mink
    • Zebrafish (Danio rerio)
  • Images available
  • Analytical Method
    • Semi-Quantitative
    • Quantitative
  • Detection Method
    • Fluorometric
  • Plate
    • Without plate
  • Binding Specificity
    • total
    • pSer133
    • pSer15
    • pSer209
    • pSer218
    • pSer222
    • pSer235
    • pSer236
    • pSer240
    • pSer244
    • pSer376
    • pSer473
    • pSer51
    • pSer536
    • pSer9
    • pThr180
    • pThr183
    • pThr202
    • pThr308
    • pThr37
    • pThr389
    • pThr46
    • pTyr1068
    • pTyr1234
    • pTyr1235
    • pTyr182
    • pTyr185
    • pTyr204
    • pTyr641
    • pTyr694
    • pTyr705
    • pSer112
    • pSer465
    • pSer467
    • pTyr1150
    • pTyr1151
    • pTyr419
  • Sample
    • Cell Lysate
    • Cell Culture Supernatant
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