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TR-FRET Assays

TR-FRET (Time-Resolved Fluorescence Energy Transfer) is a special form of FRET (Förster Resonance Energy Transfer) in which the long-lived emission fluorophores are used as donors. Sandwich TR-FRET immunoassays for semi-quantitative detection enable the examination of phosphorylation events in cellular signaling pathways. We offer high quality TR-FRET Assay Kit alternatives at an outstanding price.

TR-FRET Method and Products - antibodies-online.com
  • Unlike other TR-FRET kits, we include a positive control in every kit to allow results you can depend on.
  • Our Phospho-Protein Kits enable the detection of proteins phosphorylated at defined residues.
  • Total-Protein Kits allow the detection of total protein in a sample and can be used to normalize your data obtained with the Phospho-Kits.
  • The Phospho- and Total Protein Kits are designed for simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit.

Our kits come in convenient sizes of 100 and 500 points for 69 key targets.

Highly validated components and Data-transparency

Comprehensive Technical Data Sheets showing the pharmacological validation of each assay kit are available. Browse our available TR-FRET assays down below! Our support team is happy to help you select the proper kit for your research. We can answer any questions you may have. Please contact us via phone, email or use our live chat system.

All TR-FRET Assay Products

68 results:

Phospho-Met (Y1234/1235) and Total MET TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6939009
$1,476.00
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-4EBP1 (T37/T46) and Total 4EBP1 TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Monkey, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938945
$1,476.00
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-CREB (S133) and Total CREB TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938956
$1,476.00
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-BAD (S112) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Monkey, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938981
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-Met (Y1234/1235) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6939010
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-4EBP1 (T37/T46) TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Monkey, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938946
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-CREB (S133) TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938957
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Total Met TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6939011
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Total 4EBP1 TR-FRET Cellular Assay Kit Kit

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Monkey, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938947
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Total CREB TR-FRET Cellular Assay Kit Kit

Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938958
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-SMAD2 (S465/S467) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938968
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-STAT6 (Y641) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938970
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Total STAT6 TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938971
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-Ribosomal Protein S6 (S235/236) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938972
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days

Phospho-Ribosomal Protein S6 (S240/244) TR-FRET Cellular Assay Kit Kit

CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Semi-Quantitative Cell Lysate
Catalog No. ABIN6938974
$436.00
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
  • Type Kit
    • Kit
  • Target
    • Ribosomal Protein S6 (RPS6)
    • V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
    • Epidermal Growth Factor Receptor (EGFR)
    • Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A)
    • Eukaryotic Translation Initiation Factor 4E (EIF4E)
    • Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)
    • Glycogen Synthase Kinase 3 beta (GSK3b)
    • Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)
    • Met Proto-Oncogene (MET)
    • Mitogen-Activated Protein Kinase 1/3 (MAPK1/3)
    • Mitogen-Activated Protein Kinase 8 (MAPK8)
    • Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
    • Nuclear Factor kappa B (NFkB)
    • Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)
    • STAT5 A/B
    • Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
    • Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6)
    • Tumor Protein P53 (TP53)
    • cAMP Responsive Element Binding Protein 1 (CREB1)
    • BCL2-Associated Agonist of Cell Death (BAD)
    • Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
    • Insulin Receptor beta (ISR-beta)
    • Mitogen-Activated Protein Kinase 14 (MAPK14)
    • Proto-oncogene tyrosine-protein kinase Src (Src)
    • SMAD, Mothers Against DPP Homolog 2 (SMAD2)
    • p38 alpha beta gamma
  • Application Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • ELISA
    • Biochemical Assay (BCA)
    • Functional Studies (Func)
    • Activity Assay (AcA)
    • Detection (D)
    • Quantification (Q)
    • Western Blotting (WB)
    • Enzyme Activity Assay (EAA)
    • Cellular Assay (CA)
    • Labeling (Lbl)
    • Screening Assay (ScA)
    • Flow Cytometry (FACS)
    • ELISpot
    • Conjugation (Con)
    • Chromatin Immunoprecipitation (ChIP)
    • Fluorescence Resonance Energy Transfer Microscopy (FRET)
    • Purification (Purif)
    • Binding Studies (Bind)
    • Immunoprecipitation (IP)
    • Immunofluorescence (IF)
    • Immunohistochemistry (IHC)
    • Apoptosis Detection (AD)
    • Mass Spectrometry (MS)
    • Inhibition Assay (InhA)
    • Colloidal Gold Immunochromatography Assay (GICA)
    • Immunocytochemistry (ICC)
    • Protein Complex Immunoprecipitation (Co-IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • High Pressure Liquid Chromatography (HPLC)
    • Pull-Down Assay (Pull-Down)
    • Affinity Measurement (AM)
    • Fluorescence Microscopy (FM)
    • Activation (Act)
    • Immunoassay (IA)
    • Cell Viability Assay (CVA)
    • High-throughput Screening (HTS)
    • Rapid Test (rapid)
    • Cytotoxicity Test (CyTox)
    • Proliferation Assay (ProA)
    • Isotyping (IsoT)
    • Staining Methods (StM)
    • Separation (Sep)
    • Cell Culture (CC)
    • ChIP DNA-Sequencing (ChIP-seq)
    • Light Microscopy (LM)
    • Sample Preparation (Prep)
    • ChIP quantitative PCR (ChIP-qPCR)
    • Protein Extraction (PEx)
      Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Monkey
    • Cow
    • Dog
    • Hamster
    • Pig
    • Mink
    • Zebrafish (Danio rerio)
  • Images available
  • Analytical Method
    • Semi-Quantitative
  • Detection Method
    • Fluorometric
  • Binding Specificity
    • total
    • pSer133
    • pSer15
    • pSer209
    • pSer218
    • pSer222
    • pSer235
    • pSer236
    • pSer240
    • pSer244
    • pSer376
    • pSer473
    • pSer51
    • pSer536
    • pSer9
    • pThr180
    • pThr183
    • pThr202
    • pThr308
    • pThr37
    • pThr389
    • pThr46
    • pTyr1068
    • pTyr1234
    • pTyr1235
    • pTyr182
    • pTyr185
    • pTyr204
    • pTyr641
    • pTyr694
    • pTyr705
    • pSer112
    • pSer465
    • pSer467
    • pTyr1150
    • pTyr1151
    • pTyr419
  • Sample
    • Cell Lysate
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