TR-FRET Assays

TR-FRET (Time-Resolved Fluorescence Energy Transfer) is a special form of FRET (Förster Resonance Energy Transfer) in which the long-lived emission fluorophores are used as donors.

For the investigation of protein interactions, methods have been established which exploit the energy transfer from fluorescence light to a second fluorophore (FRET). This only takes place when two molecules are in close proximity, for example when two proteins interact. This requires two proteins that are coupled to different fluorophores: a donor that emits light after excitation and only excites the acceptor fluorophore when the bound proteins interact. The ratio of acceptor to donor fluorescence provides information on the association of both proteins.

Time Resolved FRET Cellular Kinase Assay

The TR-FRET Cellular Kinase Assay Kits are intended for the simple and rapid detection of either phosphorylated or total protein in cell lysates using the homogeneous (no-wash) TR-FRET technology. Cells are first treated to modulate protein levels. Cells are then lysed with the specific lysis buffer provided in the kit and the target protein is subsequently detected in one-step using the TR-FRET reagents. The target protein (phosphorylated or total) is detected in a sandwich assay format using 2 different labelled specific antibodies.

Note: The availability of two different antibody pairs allow the simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit.

The transmitter of the fluorescence emission is preferably made up of molecules with a long emission lifetime (time resolved FRET, TR-FRET). This allows the signal to be detected at a later point in time and over a longer period of time and minimizes background fluorescence. Excitation of the donor with a flash lamp (320 or 340 nm) or a laser (337 nm) triggers a Fluorescence Resonance Energy Transfer (FRET) towards the acceptor, which in turn emits light at 665 nm. The signal at 665 nm is proportional to the concentration of protein in the cell lysate.

Browse our available TR-FRET assays down below! Our support team will gladly help you with finding the right kit for your needs or answer occuring questions. Please contact us via phone, email or use our live chat system.

Type
  • 67
    Kit
Antigen
  • 5
  • 5
  • 3
  • 3
  • 3
Reactivity
  • 67
  • 50
  • 46
  • 13
  • 2
Application
  • 115699
  • 600
  • 516
  • 379
  • 266
Analytical Method
  • 67
Detection Method
  • 67
Binding Specificity
  • 40
  • 2
  • 2
  • 2
  • 2
Sample
  • 67
67 Products
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Monkey, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938947
» Show selected EIF4EBP1 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Monkey, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938981
» Show selected BAD Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938957
» Show selected CREB1 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938958
» Show selected CREB1 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6939010
» Show selected MET Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938949
» Show selected NFkB Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938950
» Show selected NFkB Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6939011
» Show selected MET Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Monkey, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938944
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938963
» Show selected TP53 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938964
» Show selected TP53 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938972
» Show selected RPS6 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938974
» Show selected RPS6 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938976
» Show selected RPS6 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6939000
» Show selected LCP2 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Monkey, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938946
» Show selected EIF4EBP1 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6939001
» Show selected LCP2 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938968
» Show selected SMAD2 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6939005
» Show selected SRC Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938991
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938992
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938970
» Show selected STAT6 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938971
» Show selected STAT6 Kits with more information such as references or interaction partners.
$595.00
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938956
» Show selected CREB1 Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6939009
» Show selected MET Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938948
» Show selected NFkB Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938962
» Show selected TP53 Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938975
» Show selected RPS6 Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
ReactivityHuman, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938973
» Show selected RPS6 Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
Semi-Quantitative, Fluorometric, Samples: Cell Lysate
ReactivityHuman, Monkey, Mouse (Murine), Rat (Rattus)
Detection MethodFluorometric,
Detection RangeSemi-Quantitative
Order details Cat. No. ABIN6938945
» Show selected EIF4EBP1 Kits with more information such as references or interaction partners.
$1,895.00
500 points
Plus shipping costs $45.00
Delivery in 2 to 4 Business Days
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