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TR-FRET Assays

TR-FRET (Time-Resolved Fluorescence Energy Transfer) is a special form of FRET (Förster Resonance Energy Transfer) in which the long-lived emission fluorophores are used as donors. Sandwich TR-FRET immunoassays for semi-quantitative detection enable the examination of phosphorylation events in cellular signaling pathways. We offer high quality TR-FRET Assay Kit alternatives at an competitive price. Our kits come in convenient sizes of 100 and 500 points for 69 key targets.

TR-FRET Method and Products - antibodies-online.com

Advantages of TR-FRET Assays

TR-FRET Asasays are a truly homogeneous assay technology platform. The technique combines the robust FRET approach with the sensitivity of TRF and is often utilized in high throughput screening and drug discovery. However the assay is also gaining popularity in basic research. In comparison with ELISA and WB, TR-FRET offers distinct advantages:

  • No washing steps
  • Single incubation step
  • Save hands on time
  • Increase throughput
  • Increase reproducibility

Phospho-Protein TR-FRET Assays

Our Phospho-Protein Kits enable the detection of proteins phosphorylated at defined residues. Total-Protein Kits allow the detection of total protein in a sample and can be used to normalize your data obtained with the Phospho-Kits. The Phospho- and Total Protein Kits are designed for simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit. The assays are compatible with both adherent and suspension cells, and are conducted in just 3 steps. Cells are first treated to modulate protein levels. Cells are then lysed with the specific lysis buffer provided in the kit and the target protein is subsequently detected in one-step using the TR-FRET reagents. The Phospho- and Total Protein Kits are designed for simultaneous measurement of two proteins (phosphorylated and total) in separate wells from the same cell lysate using a single kit.

  • Simultaneous measurement of two proteins in separate wells from the same cell lysate using a single kit.
  • 3 steps only
  • Compatible with both adherent and suspension cells
  • Positive control in both kit types included

New Cytokine TR-FRET Assays Available

antibodies-online now offers assays for reliable detection of cytokines. The simple, rapid and sensitive immunoassay is based on the homogeneous (no-wash) TR-FRET technology. The assays are easy to perform with only 4 steps and 1 h incubation in total. A distinct advantage of the TR-FRET technology is that it does not require addition of potassium fluoride to ensure signal stability. The non-toxic nature of makes it an ideal candidate for multiplexing with other assay technologies using a sequential assay protocol.

Product
Sample Type
Analytical Method
Detection Range
Cat. No.
Quantity
Datasheet
Sample Type Cell Culture Supernatant
Analytical Method Quantitative
Detection Range 21 pg/mL - 30000 pg/mL
Cat. No. ABIN6999323
Quantity 100 points
Sample Type Cell Culture Supernatant
Analytical Method Quantitative
Detection Range 26 pg/mL - 10000 pg/mL
Cat. No. ABIN6999324
Quantity 100 points
Sample Type Cell Culture Supernatant
Analytical Method Quantitative
Detection Range 10 pg/mL - 100000 pg/mL
Cat. No. ABIN6999325
Quantity 100 points
Sample Type Cell Culture Supernatant
Analytical Method Quantitative
Detection Range 35 pg/mL - 30000 pg/mL
Cat. No. ABIN6999326
Quantity 100 points
Sample Type Cell Culture Supernatant
Analytical Method Quantitative
Detection Range 15 pg/mL - 10000 pg/mL
Cat. No. ABIN6999327
Quantity 100 points
Sample Type Cell Culture Supernatant
Analytical Method Quantitative
Detection Range 13 pg/mL - 30000 pg/mL
Cat. No. ABIN6999322
Quantity 100 points

Highly validated components and Data-transparency

Comprehensive Technical Data Sheets showing the pharmacological validation of each assay kit are available. Browse our available TR-FRET assays down below! Our support team is happy to help you select the proper kit for your research. We can answer any questions you may have. Please contact us via phone, email or use our live chat system.

74 results:

Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat, Monkey Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938945
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938956
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938969
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938973
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938975
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938990
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
CA, TR-FRET Reactivity: Human Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6939009
$1,196.84
Plus shipping costs $45.00
500 points
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
Synonym: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat, Monkey Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938946
$353.25
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
Synonym: cAMP Responsive Element Binding Protein 1 CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938957
$353.25
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
CA, TR-FRET Reactivity: Human, Mouse, Rat Fluorometric Cell Lysate Semi-Quantitative
Catalog No. ABIN6938974
$353.25
Plus shipping costs $45.00
100 points (More quantities available)
local_shipping Shipping to: United States
Delivery in 2 to 4 Business Days
  • Type Kit
    • Kit
  • Target
    • Ribosomal Protein S6 (RPS6)
    • V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
    • Epidermal Growth Factor Receptor (EGFR)
    • Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A)
    • Eukaryotic Translation Initiation Factor 4E (EIF4E)
    • Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)
    • Glycogen Synthase Kinase 3 beta (GSK3b)
    • Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)
    • Met Proto-Oncogene (MET)
    • Mitogen-Activated Protein Kinase 1/3 (MAPK1/3)
    • Mitogen-Activated Protein Kinase 8 (MAPK8)
    • Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
    • Nuclear Factor kappa B (NFkB)
    • Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1)
    • STAT5 A/B
    • Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3)
    • Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6)
    • Tumor Protein P53 (TP53)
    • cAMP Responsive Element Binding Protein 1 (CREB1)
    • BCL2-Associated Agonist of Cell Death (BAD)
    • Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
    • Insulin Receptor beta (ISR-beta)
    • Interferon gamma (IFNG)
    • Interleukin 1, beta (IL1B)
    • Interleukin 2 (IL2)
    • Interleukin 6 (IL6)
    • Interleukin 8 (IL8)
    • Mitogen-Activated Protein Kinase 14 (MAPK14)
    • Proto-oncogene tyrosine-protein kinase Src (Src)
    • SMAD, Mothers Against DPP Homolog 2 (SMAD2)
    • Tumor Necrosis Factor alpha (TNF alpha)
    • p38 alpha beta gamma
  • Application Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • Time-resolved Fluorescence Energy Transfer (TR-FRET)
    • ELISA
    • Functional Studies (Func)
    • Biochemical Assay (BCA)
    • Quantification (Q)
    • Labeling (Lbl)
    • Activity Assay (AcA)
    • ELISpot
    • Cellular Assay (CA)
    • Enzyme Activity Assay (EAA)
    • Flow Cytometry (FACS)
    • Fluorescence Resonance Energy Transfer Microscopy (FRET)
    • Detection (D)
    • Western Blotting (WB)
    • Inhibition Assay (InhA)
    • Colloidal Gold Immunochromatography Assay (GICA)
    • Immunocytochemistry (ICC)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Apoptosis Detection (AD)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunohistochemistry (IHC)
    • High Pressure Liquid Chromatography (HPLC)
    • Screening Assay (ScA)
    • Immunofluorescence (IF)
    • Chromatin Immunoprecipitation (ChIP)
    • Activation (Act)
    • Rapid Test (rapid)
    • Purification (Purif)
    • Immunoassay (IA)
    • Cell Viability Assay (CVA)
    • Conjugation (Con)
    • Immunoprecipitation (IP)
    • Isotyping (IsoT)
    • Proliferation Assay (ProA)
    • Cytotoxicity Test (CyTox)
    • Immunostaining (ISt)
    • Protein Extraction (PEx)
    • Substrate (S)
    • BioImaging (BI)
    • Cell Culture (CC)
    • Multiplex Assay (MA)
    • Standard (STD)
    • Methylated DNA Immunoprecipitation (MeDIP)
    • Two Dimensional Difference Gel Electrophoresis (2D-DIGE)
    • ChIP DNA-Sequencing (ChIP-seq)
    • Endotoxin Detection Assay (EDA)
    • Sample Extraction (SEx)
    • SDS-PAGE (SDS)
    • Transfected Cell Culture (TCC)
    • Affinity Chromatography (AC)
    Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Monkey
    • Cow
    • Dog
    • Hamster
    • Pig
    • Mink
    • Zebrafish (Danio rerio)
  • Images available
  • Analytical Method
    • Semi-Quantitative
    • Quantitative
  • Detection Method
    • Fluorometric
  • Plate
    • Without plate
  • Binding Specificity
    • total
    • pSer133
    • pSer15
    • pSer209
    • pSer218
    • pSer222
    • pSer235
    • pSer236
    • pSer240
    • pSer244
    • pSer376
    • pSer473
    • pSer51
    • pSer536
    • pSer9
    • pThr180
    • pThr183
    • pThr202
    • pThr308
    • pThr37
    • pThr389
    • pThr46
    • pTyr1068
    • pTyr1234
    • pTyr1235
    • pTyr182
    • pTyr185
    • pTyr204
    • pTyr641
    • pTyr694
    • pTyr705
    • pSer112
    • pSer465
    • pSer467
    • pTyr1150
    • pTyr1151
    • pTyr419
  • Sample
    • Cell Lysate
    • Cell Culture Supernatant
  • Supplier
    • antibodies-online
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