CD82 (human): 293T Lysate
CD82
WB
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
1 image
(6 references)-
- Target See all CD82 products
- CD82
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB)
- Characteristics
- CD82 (KAI1) is a highly glycosylated membrane protein belonging to the tetraspanins. The tetraspanins are a family of proteins that possesses four transmembrane domains, namely, the NH2- and COOH-terminal cytoplasmic domains, and two extracellular loops. A few tetraspanins, including CD151, tetraspanin 8, CD82, and CD9, are involved in tumor metastasis. CD82 is a metastasis suppressor implicated in cell fusion, adhesion, migration, apoptosis, and cell morphology alterations. It has attracted much attention in various human cancers, such as prostate, pancreatic, lung, gastric, hepatic, and colorectal cancers. As a membrane protein, CD82 interacts with integrins, other tetraspanins (CD9, CD63, and CD81), cell-surface molecules (CD4, CD8, CD19, and CD21), and MHC class I and class II, which includes CD82 into a newly defined tetraspanin web Two types of PTMs, namely, glycosylation and palmitoylation, occur on CD82. The palmitoylation is reportedly an important PTM for its motility- and invasiveness-inhibitory activity. On the other hand, CD82 has three potential N-glycosylation sites located at the large extracellular loop. CD82N- glycosylation has been previously shown to play a potent role in cell adhesion and motility. White et al. found that CD82 has distinct heterogeneous protein band patterns in various human cancer cell lines, which are most likely associated with N-glycosylation. Lysate of human CD82 transfected 293T cells
- Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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CD82 293T Cell Transient Overexpression Lysate(Denatured)
CD82 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- CD82 (h): 293T Lysate is suitable as a Western Blotting positive control for human reactive CD82 antibodies. Recommended use: 10-20 µl per lane. Control 293T Lysate: 293LYS is available as a Western Blotting negative control lysate derived from non-transfected 293T cells.
- Restrictions
- For Research Use only
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- Handling Advice
- Repeated freezing and thawing should be minimized. Sample vial should be boiled once prior to use. Non-hazardous. No MSDS required.
- Storage
- -20 °C
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: "Closer association of IA-2 humoral autoreactivity with HLA DR3/4 than DQB1*0201/*0302 in Korean T1D patients." in: Annals of the New York Academy of Sciences, Vol. 1037, pp. 104-9, (2005) (PubMed).
: "Association of HLA-DR, -DQ genotype and CTLA-4 gene polymorphism with Graves' disease in Japanese children." in: Hormone research, Vol. 63, Issue 2, pp. 55-60, (2005) (PubMed).
: "Distinct tumour necrosis factor alpha, interferon gamma, interleukin 10, and cytotoxic T cell antigen 4 gene polymorphisms in disease occurrence and end stage renal disease in Wegener's granulomatosis." in: Annals of the rheumatic diseases, Vol. 64, Issue 3, pp. 457-61, (2005) (PubMed).
: "Association of Helicobacter pylori-related distal gastric cancer with the HLA class II gene DQB10602 and cagA strains in a southern European population." in: Helicobacter, Vol. 10, Issue 1, pp. 12-21, (2005) (PubMed).
: "Allelic polymorphism in introns 1 and 2 of the HLA-DQA1 gene." in: Tissue antigens, Vol. 65, Issue 1, pp. 56-66, (2005) (PubMed).
: "Anti-HLA class II antibodies in kidney retransplant patients." in: Tissue antigens, Vol. 65, Issue 4, pp. 370-8, (2005) (PubMed).
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: "Closer association of IA-2 humoral autoreactivity with HLA DR3/4 than DQB1*0201/*0302 in Korean T1D patients." in: Annals of the New York Academy of Sciences, Vol. 1037, pp. 104-9, (2005) (PubMed).
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- Target
- CD82
- Alternative Name
- CD82 (CD82 Products)
- Synonyms
- AA682076 Lysate, AL023070 Lysate, C33 Lysate, IA4 Lysate, Kai1 Lysate, Tspan27 Lysate, 4F9 Lysate, GR15 Lysate, KAI1 Lysate, R2 Lysate, SAR2 Lysate, ST6 Lysate, TSPAN27 Lysate, CD82 antigen Lysate, CD82 molecule Lysate, Cd82 molecule Lysate, Cd82 Lysate, CD82 Lysate
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