AHNAK HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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- Target See all AHNAK products
- AHNAK (AHNAK Nucleoprotein (AHNAK))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
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AHNAK HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-AHNAK full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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AHNAK 293T Cell Transient Overexpression Lysate(Denatured)
AHNAK WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
AHNAK 293T Cell Transient Overexpression Lysate(Denatured)AHNAK WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Product Quality tested by: Transient overexpression cell lysate was tested with Anti-AHNAK antibody by Western Blots.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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- Target
- AHNAK (AHNAK Nucleoprotein (AHNAK))
- Alternative Name
- AHNAK (AHNAK Products)
- Synonyms
- AHNAKRS Lysate, 1110004P15Rik Lysate, 2310047C17Rik Lysate, AA589382 Lysate, AV091586 Lysate, DY6 Lysate, AHNAK 1 Lysate, RGD1308572 Lysate, UV118 Lysate, AHNAK Lysate, wu:fb05a01 Lysate, wu:fu74f12 Lysate, LOC733715 Lysate, AHNAK nucleoprotein Lysate, AHNAK nucleoprotein (desmoyokin) Lysate, AHNAK nucleoprotein pseudogene Lysate, AHNAK Lysate, Ahnak Lysate, ahnak Lysate, LOC748622 Lysate
- Background
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Full Gene Name: AHNAK nucleoprotein
Synonyms: AHNAKRS,MGC5395 - Gene ID
- 79026
- NCBI Accession
- NP_076965, NM_024060
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