CBS HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
CBS
WB, IP
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
Reactivity: Human
-
- Target See all CBS products
- CBS (Cystathionine-beta-Synthase (CBS))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
CBS HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-CBS full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
CBS 293T Cell Transient Overexpression Lysate(Denatured)
CBS WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
-
- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-CBS antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- CBS (Cystathionine-beta-Synthase (CBS))
- Alternative Name
- CBS (CBS Products)
- Synonyms
- hip4 Lysate, GB12529 Lysate, CBS Lysate, DDBDRAFT_0189727 Lysate, DDBDRAFT_0191292 Lysate, DDB_0189727 Lysate, DDB_0191292 Lysate, cbs Lysate, AI047524 Lysate, AI303044 Lysate, HIP4 Lysate, cb442 Lysate, wu:fb37g05 Lysate, wu:fm61c07 Lysate, wu:fq06c06 Lysate, zgc:113704 Lysate, cystathionine-beta-synthase L homeolog Lysate, cystathionine-beta-synthase Lysate, cystathionine beta-synthase Lysate, cystathionine beta-synthase CBS Lysate, cystathionine beta synthase Lysate, cystathionine-beta-synthase S homeolog Lysate, cystathionine-beta-synthase b Lysate, cbs.L Lysate, cbs Lysate, Cbs Lysate, CBS Lysate, CNA06170 Lysate, Tb11.02.5400 Lysate, cysB Lysate, LOC100635325 Lysate, cbs.S Lysate, cbsb Lysate
- Background
-
Full Gene Name: cystathionine-beta-synthase
Synonyms: HIP4 - Gene ID
- 875
- NCBI Accession
- NP_000062, NM_000071
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