bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
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- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Whole cell lysate
- Specificity
- Non-denatured
- Characteristics
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b mMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
Used Tissue: Bone Marrow - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Whole Cell Lysate
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- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
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Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
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Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).
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Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).
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- Background
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Full Gene Name:
Synonyms:
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