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bmMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)

IP, WB Whole Cell Lysate Cell Lysate HEK 293T Cells Reactivity: Human
Catalog No. ABIN1339644
  • Protein Species
    Human
    Species of Lysate
    Human Cells
    Application
    Immunoprecipitation (IP), Western Blotting (WB)
    Purpose
    Whole cell lysate
    Specificity
    Non-denatured
    Characteristics
    b mMSCs(human bone marrow-derived mesenchymal stem cell) whole cell lysate (non-denatured)
    Used Tissue: Bone Marrow
    Lysed Cells
    HEK 293T Cells
    Lysate Type
    Cell Lysate
    Lysate Fraction
    Whole Cell Lysate
  • Application Notes
    Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
    Comment

    Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).
    Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
    Handling Advice
    Aliquot to avoid repeated freezing and thawing.
    Storage
    -80 °C
    Storage Comment
    Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
  • Chang, Shih, Tseng, Hsieh, Lee, Hwang: "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood." in: Stem cells (Dayton, Ohio), Vol. 24, Issue 3, pp. 679-85, (2006) (PubMed).

  • Background
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