GBA HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
-
- Target See all GBA products
- GBA (Glucosidase, Beta, Acid (GBA))
- Protein Species
- Human
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB), Immunoprecipitation (IP)
- Purpose
- Transient Overexpression Lysate
- Specificity
- Non-denatured
- Characteristics
-
GBA HEK293 Cell Transient Overexpression Lysate (Non-Denatured)
Used Plasmid: pCMV-GBA full length - Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
-
GBA 293T Cell Transient Overexpression Lysate(Denatured)
GBA WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
GBA 293T Cell Transient Overexpression Lysate(Denatured)GBA WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
-
- Application Notes
- Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95 °C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
- Comment
-
Product Quality tested by: Transient overexpression cell lysate was tested with Anti-GBA antibody by Western Blots.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2 mg/mL
- Buffer
- Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % SDS, 1 % Sodium deoxycholate, 1 mM PMSF.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
-
- Target
- GBA (Glucosidase, Beta, Acid (GBA))
- Alternative Name
- GBA (GBA Products)
- Synonyms
- GBA1 Lysate, GCB Lysate, GLUC Lysate, BETA-GLUCOSIDASE Lysate, T12J13.8 Lysate, T12J13_8 Lysate, beta glucosidase 25 Lysate, PSPTO3318 Lysate, PSPTO4290 Lysate, GC Lysate, GCase Lysate, betaGC Lysate, glucosylceramidase beta Lysate, beta glucosidase 25 Lysate, beta-glucosidase Lysate, Beta-glucosidase Lysate, glucosidase, beta, acid Lysate, glucosylceramidase Lysate, bglX-2 Lysate, GBA Lysate, BGLU25 Lysate, PSPTO_3318 Lysate, bglX Lysate, bglA4 Lysate, Gba Lysate, LOC100399524 Lysate, bglA.2 Lysate, bglX-2 Lysate
- Background
-
Full Gene Name: glucosidase, beta, acid (includes glucosylceramidase)
Synonyms: GBA1,GCB,GLUC - Gene ID
- 2629
- NCBI Accession
- NP_000148, NM_000157
-
