Multiple Tag Cell Lysate

Details for Product No. ABIN1536505, Supplier: Log in to see
Protein Species
Escherichia coli (E. coli)
Species of Lysate
Human Cells
Application
Dot Blot (DB), Western Blotting (WB)
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Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Lysed Cells HEK 293T Cells
Background Epitope Tag Positive Control Lysates. Multiple Tag Cell Lysate is whole E. coli cell lysate comprised of about 30% specially designed fusion protein, which act as a positive control for Western blot or Dot blot. The fusion protein is a 52 kD protein containing 16 commonly used tags, which are, in order, Trx, T7, HSV, C-Myc, SVS-g, Glu-Glu, V5, E tag, DYKDDDDK, S-tag, HA, KT3, E2, AU1, AU5, and His tag.
Application Notes Positive control for Western blot and Dot blot.
Restrictions For Research Use only
Concentration 200 µg of whole E. coli cell lysate (lyophilized) Volume Add 200 µl of ddH2O to make the final of 1 µg/µl in 1X SDS Loading Buffer. 0.02 - 0.5 µl of the lysate is recommended for each lane of a minigel. For accurate loading, dilute with 1X SDS Loading Buf
Storage -20 °C
Storage Comment Store at -20°C or below. After reconstitution, aliquot and store at -20°C or below.
Product cited in: Lawless, Mankan, White, ODwyer, Norris: "Expression of hereditary hemochromatosis C282Y HFE protein in HEK293 cells activates specific endoplasmic reticulum stress responses." in: BMC cell biology, Vol. 8, pp. 30, 2007 (PubMed).