KRT6A 293 Cell Lysate

Details for Product No. ABIN1602185, Supplier: Log in to see
Protein
  • CK6A
  • CK6C
  • CK6D
  • K6A
  • K6C
  • K6D
  • KRT6C
  • KRT6D
  • 2310016L08Rik
  • Krt2-6a
  • Krt2-6c
  • MK6a
  • mK6[a]
  • CK-6A
  • Krt6a
  • KRT6A
  • keratin 6A
  • keratin 6C
  • similar to keratin complex 2, basic, gene 6a
  • keratin, type II cytoskeletal 6A
  • KRT6A
  • Krt6a
  • KRT6C
  • LOC683313
  • LOC101112304
Protein Species
Human
Species of Lysate
Human Cells
Conjugate
Myc-DYKDDDDK Tag
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Brand TACL™
Characteristics Antigen standard for keratin 6A (KRT6A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Lysed Cells HEK 293T Cells
Alternative Name KRT6A
Background Alternate Names: keratin, epidermal type II, K6A, cytokeratin 6A, 56 cytoskeletal type II keratin, keratin 6C, keratin, epidermal type II, K6C, cytokeratin 6C, type II keratin isoform K6c, keratin, type II cytoskeletal 6D, cytokeratin 6D, K6D keratin, CK-6A, CK-6D, kera, KRT6A, CK6A, CK6C, CK6D, K6A, K6C, K6D, KRT6C, KRT6D
NCBI Accession NP_005545
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -20 °C,-80 °C
Storage Comment Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Expiry Date 6 months