GUCY2C 293 Cell Lysate

Details for Product No. ABIN1602384, Supplier: Log in to see
Protein
  • DIAR6
  • GUC2C
  • MECIL
  • MUCIL
  • STAR
  • AI893437
  • GC-C
  • Gcc
  • GCC
  • gucy2c-A
  • GUCY2C
  • Gucy2c
  • guanylate cyclase 2C
  • guanylate cyclase 2c
  • guanylate cyclase 2C L homeolog
  • GUCY2C
  • Gucy2c
  • gucy2c.L
  • gucy2c
Protein Species
Human
Species of Lysate
Human Cells
Conjugate
Myc-DYKDDDDK Tag
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Options
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Brand TACL™
Characteristics Antigen standard for guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC213901, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Lysed Cells HEK 293T Cells
Alternative Name GUCY2C
Background Alternate Names: guanylyl cyclase C, GC-C, hSTAR, STA receptor, intestinal guanylate cyclase, heat-stable enterotoxin receptor, GUCY2C, GUC2C, STAR
NCBI Accession NP_004954
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -20 °C,-80 °C
Storage Comment Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Expiry Date 6 months