UBE2M 293 Cell Lysate

Details for Product No. ABIN1602673, Supplier: Log in to see
Protein
  • DDBDRAFT_0204633
  • DDBDRAFT_0238042
  • DDB_0204633
  • DDB_0238042
  • UBC-RS2
  • UBC12
  • hUbc12
  • 2510040H03Rik
  • Ubc-rs2
  • ubiquitin conjugating enzyme E2M
  • ubiquitin-conjugating enzyme E2M
  • ubiquitin conjugating enzyme E2 M
  • NEDD8-conjugating enzyme
  • ubiquitin conjugating enzyme E2M S homeolog
  • ube2m
  • LOC692934
  • UBE2M
  • Ube2m
  • ube2m.S
Protein Species
Human
Species of Lysate
Human Cells
Conjugate
Myc-DYKDDDDK Tag
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
Options
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Brand TACL™
Characteristics Antigen standard for ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) (UBE2M) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
Lysate Fraction Whole Cell Lysate
Lysate Type Overexpression Lysate
Lysed Cells HEK 293T Cells
Alternative Name UBE2M
Background Alternate Names: ubiquitin carrier protein M, yeast UBC12 homolog, nedd8-conjugating enzyme Ubc12, ubiquitin-protein ligase M, NEDD8 protein ligase, NEDD8 carrier protein, ubiquitin-conjugating enzyme E2 M, UBE2M, hUbc12, UBC-RS2, UBC12
NCBI Accession NP_003960
Application Notes WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 μg lysate per lane.
Restrictions For Research Use only
Format Liquid
Buffer Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
Handling Advice Minimize freeze-thaw cycles.
Storage -20 °C,-80 °C
Storage Comment Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Expiry Date 6 months